LOCUS       Exported               13893 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLMB51, complete sequence.
ACCESSION   JQ895026
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS.
  TITLE     Regulatable vectors for environmental gene expression in 
            alphaproteobacteria
  JOURNAL   Appl. Environ. Microbiol. 78 (19), 7137-7140 (2012)
  PUBMED    22820336
REFERENCE   2  (bases 1 to 13893)
  AUTHORS   Poole PS.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2012) Molecular Microbiology, John Innes Centre, 
            Colney Lane, Norwich NR47UH, UK
REFERENCE   3  (bases 1 to 13893)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Cloning vector pLMB51"
                     /mol_type="other DNA"
                     /db_xref="taxon:1200802"
     CDS             complement(733..1392)
                     /codon_start=1
                     /gene="parA"
                     /product="ParA"
                     /label=parA
                     /protein_id="AFR24674.1"
                     /translation="MATREQQPEGRRLKVARIYLRASTDEQNLERQESLVAATRAAGYY
                     VAGIYREKASGARADRPELLRMIADLQPGEVVVAEKIDRISRLPLAEAERLVASIRAKG
                     AKLAVPGVVDLSELAAEANGVAKIVLESVQDMLLKLALQMARDDYEDRRERQRQGVQLA
                     KAAGRYTGRKRDAGMHDRIITLRSGGSSIAKTAKLVGCSPSQVKRVWAAWNAQQQK"
     gene            complement(733..1392)
                     /gene="parA"
                     /label=parA
     CDS             complement(1353..2198)
                     /codon_start=1
                     /gene="parB"
                     /product="ParB"
                     /label=parB
                     /note="partition protein"
                     /protein_id="AFR24673.1"
                     /translation="MPARLGPNGYTQFDQGGRDEGGQALPTTSIHRLQHRGAGLRAWTR
                     RHTRPAHPRPGARGPRPARPGRVPFAGVRLAGGAGRLLQGQFRCSAAPGVPRSSAMGPR
                     MKRRSYAMLRAAAALAVLVVASPAWAELRGEVVRIIDGDTIDVLVDKQPVRVRLVDIDA
                     PEKRQAFGERARQALAGMVFRRHVLVDEKDTDRYGRTLGTVWVNMELASRPPQPRNVNA
                     AMVHQGMAWAYRFHGRAADPEMLRLEQEARGKRVGLWSDPHAVEPWKWRRESNNRRDEG
                     "
     gene            complement(1353..2198)
                     /gene="parB"
                     /label=parB
     CDS             complement(1883..2176)
                     /codon_start=1
                     /gene="parC"
                     /product="ParC"
                     /label=parC
                     /note="partition protein"
                     /protein_id="AFR24675.1"
                     /translation="MGIPNLTKGDAMRAAKHYRQLLSIDFNIEALAFVPGPDGTRGRRI
                     HVLGREVRDRPGLVEYLSPAFGSRVALDGYCKANFDAVLHLAYPDHQQWGHA"
     gene            complement(1883..2176)
                     /gene="parC"
                     /label=parC
     CDS             2327..2578
                     /codon_start=1
                     /gene="parD"
                     /product="ParD"
                     /label=parD
                     /note="partition protein"
                     /protein_id="AFR24676.1"
                     /translation="MSRLTIDMTDQQHQSLKALAALQGKTIKQYALERLFPGDADADQA
                     WQELKTMLGNRINDGLAGKVSTKSVGEILDEELSGDRA"
     gene            2327..2578
                     /gene="parD"
                     /label=parD
     CDS             2575..2886
                     /codon_start=1
                     /gene="parE"
                     /product="ParE"
                     /label=parE
                     /note="partition protein"
                     /protein_id="AFR24677.1"
                     /translation="MTAYILTAEAEADLRGIIRYTRREWGAAQVRRYIAKLEQGIARLA
                     AGEGPFKDMSELFPALRMARCEHHYVFCLPRAGEPALVVAILHERMDLMTRLADRLKG"
     gene            2575..2886
                     /gene="parE"
                     /label=parE
     primer_bind     complement(3289..3305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3313..3329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3337..3367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3382..3403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     oriT            3594..3702
                     /note="incP origin of transfer"
     promoter        3885..3989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3990..4889
                     /codon_start=1
                     /gene="Bla"
                     /product="beta-lactamase"
                     /label=Bla
                     /protein_id="AFR24678.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITIADNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHWVTVRPSLLIYTLD"
     gene            3990..4889
                     /gene="Bla"
                     /label=Bla
     CDS             3990..4847
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITIADNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(5370..6644)
                     /codon_start=1
                     /gene="tetA"
                     /product="TeTA"
                     /label=tetA
                     /note="tetracycline resistance"
                     /protein_id="AFR24679.1"
                     /translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNIPLIVILSTVALDAVG
                     IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALVQFACAPVLGALSDRFGRRPILLVS
                     LAGATVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA
                     CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL
                     NPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG
                     ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI
                     GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG
                     AALYLLCLPALRRGLWSGAGQRADR"
     gene            complement(5370..6644)
                     /gene="tetA"
                     /label=tetA
     CDS             complement(5370..6569)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6648..7325
                     /codon_start=1
                     /gene="tetR"
                     /product="TeTR"
                     /label=tetR
                     /note="tetracycline resistance"
                     /protein_id="AFR24680.1"
                     /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV
                     QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD
                     GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD
                     AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK
                     GDD"
     gene            6648..7325
                     /gene="tetR"
                     /label=tetR
     CDS             6675..7325
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             9184..10332
                     /codon_start=1
                     /gene="trfA"
                     /product="TrfA"
                     /label=trfA
                     /protein_id="AFR24681.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLMHEEIVVQFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            9184..10332
                     /gene="trfA"
                     /label=trfA
     CDS             complement(10380..12191)
                     /codon_start=1
                     /gene="gusA"
                     /product="GusA"
                     /label=gusA
                     /note="beta galacturonidase"
                     /protein_id="AFR24682.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(10380..12191)
                     /gene="gusA"
                     /label=gusA
     regulatory      12241..12397
                     /regulatory_class="promoter"
                     /gene="tauAp_tauR"
     gene            12241..12397
                     /gene="tauAp_tauR"
                     /label=tauAp_tauR