LOCUS       Exported                6879 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLMB509, complete sequence.
ACCESSION   JQ895027
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS.
  TITLE     Regulatable vectors for environmental gene expression in 
            alphaproteobacteria
  JOURNAL   Appl. Environ. Microbiol. 78 (19), 7137-7140 (2012)
  PUBMED    22820336
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Tett A, Rudder S, Bourdes A, Poole P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2012) Molecular Microbiology, John Innes Centre, 
            Colney Lane, Norwich NR47UH, UK
REFERENCE   3  (bases 1 to 6879)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Cloning vector pLMB509"
                     /mol_type="other DNA"
                     /db_xref="taxon:1200803"
     CDS             171..911
                     /codon_start=1
                     /gene="gfp"
                     /product="Gfp"
                     /label=gfp
                     /note="green fluorescent protein"
                     /protein_id="AFR24683.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKHMHHHHHH"
     gene            171..911
                     /gene="gfp"
                     /label=gfp
     CDS             171..884
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             891..908
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      942..1028
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1717..2379)
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="replication protein"
                     /protein_id="AFR24684.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            complement(1717..2379)
                     /gene="rep"
                     /label=rep
     rep_origin      2380..3149
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3373..4377
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label=mob
                     /note="mobilisation protein"
                     /protein_id="AFR24685.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLAAL"
     gene            3373..4377
                     /gene="mob"
                     /label=mob
     CDS             complement(4432..4965)
                     /codon_start=1
                     /gene="acc3"
                     /product="Acc3"
                     /label=acc3
                     /note="gentamicin resistance"
                     /protein_id="AFR24686.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            complement(4432..4965)
                     /gene="acc3"
                     /label=acc3
     promoter        complement(5154..5182)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     regulatory      complement(5395..6879)
                     /regulatory_class="promoter"
                     /gene="tauA_tauR"
     gene            complement(5395..6879)
                     /gene="tauA_tauR"
                     /label=tauA_tauR