LOCUS       Exported                7232 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM53, complete sequence.
ACCESSION   AF179893
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Novel Escherichia coli strains to detect protein-protein 
            interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-1999) Biochemistry and Biophysics, Texas A & M 
            University, College Station, TX 77843-2128, USA
REFERENCE   3  (bases 1 to 7232)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Cloning vector pLM53"
                     /lab_host="Escherichia coli AG1688"
                     /mol_type="other DNA"
                     /db_xref="taxon:103609"
     CDS             2650..2841
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    2943..3083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3269..3857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4028..4888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAF01199.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4028..4888)
                     /gene="bla"
                     /label=bla
     promoter        complement(4889..4980)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      4999..5026
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      5118..5204
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     gene            complement(5500..6285)
                     /gene="cat"
                     /label=cat
     CDS             complement(5500..6159)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAF01200.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     regulatory      complement(6231..6285)
                     /regulatory_class="promoter"
                     /gene="cat"
                     /note="pL promoter"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_binding    complement(6240..6256)
                     /gene="cat"
                     /label=O-L1 repressor binding site
                     /bound_moiety="O-L1 repressor"
     misc_binding    complement(6264..6280)
                     /gene="cat"
                     /label=O-L2 repressor binding site
                     /bound_moiety="O-L2 repressor"
     promoter        6369..6397
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6586..7119
                     /codon_start=1
                     /gene="gat"
                     /product="gentamicin acetyltransferase"
                     /label=gat
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAF01201.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            6586..7119
                     /gene="gat"
                     /label=gat