LOCUS       Exported                5042 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM3, complete sequence.
ACCESSION   AF179892
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Lambda repressor fusion vectors to map protein-protein interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-1999) Biochemistry and Biophysics, Texas A & M 
            University, College Station, TX 77843-2128, USA
REFERENCE   3  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Cloning vector pLM3"
                     /lab_host="Escherichia coli AG1688"
                     /mol_type="other DNA"
                     /db_xref="taxon:103608"
     regulatory      6..47
                     /regulatory_class="promoter"
     CDS             73..462
                     /codon_start=1
                     /product="lambda repressor protein"
                     /label=lambda repressor protein
                     /note="DNA binding domain"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAF01197.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAER"
     terminator      571..618
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2103..2294
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    2396..2536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2722..3310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3421..3934
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(3976..4836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAF01198.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3976..4836)
                     /gene="bla"
                     /label=bla
     promoter        complement(4837..4941)
                     /gene="bla"
                     /label=AmpR promoter