LOCUS       Exported                6941 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pLM-ITRs, complete sequence.
ACCESSION   GQ202120
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Mei L.
  TITLE     Construction of high-efficiency baculovirus expression vector and 
            its expression in chicken cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Mei L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2009) Microbiology, Life Science, Xuefu Road, 
            Harbin, Heilongjiang 150080, China
REFERENCE   3  (bases 1 to 6941)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Baculovirus expression vector pLM-ITRs"
                     /mol_type="other DNA"
                     /db_xref="taxon:653730"
     repeat_region   32..172
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    complement(189..410)
                     /label=SV40 polyA region
                     /note="SV40 polyA region"
     polyA_signal    189..310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_RNA        complement(411..1008)
                     /label=post-transcriptional regulatory element:WPRE
                     /note="post-transcriptional regulatory element:WPRE"
     misc_feature    417..1005
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             523..534
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    complement(1009..1054)
                     /label=MCS
                     /note="MCS"
     regulatory      complement(1055..1643)
                     /regulatory_class="promoter"
                     /note="CMV"
     promoter        complement(1076..1279)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1280..1583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   join(1690..1830,32..172)
                     /label=AAV
                     /note="AAV"
     repeat_region   1690..1830
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     regulatory      1860..2087
                     /regulatory_class="promoter"
                     /note="pH"
     promoter        1960..2051
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    2094..2207
                     /label=gp64 signal peptide
                     /note="gp64 signal peptide"
     CDS             2148..2207
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     misc_feature    2208..2417
                     /note="transmembrane domain and cytoplasmic tail domain of 
                     VSV-G"
     CDS             2382..2414
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     misc_feature    2439..2679
                     /label=SV40 polyA region
                     /note="SV40 polyA region"
     polyA_signal    2545..2679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2708..2873
                     /label=Tn7L
                     /note="Tn7L"
     mobile_element  2708..2873
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3057..3512
                     /label=f1
                     /note="f1"
     promoter        3539..3643
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3644..4504
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="ACS91925.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4649..5322
                     /label=pUC
                     /note="pUC"
     rep_origin      4675..5263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5566..5790
                     /label=Tn7R
                     /note="Tn7R"
     mobile_element  5566..5790
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5857..6390)
                     /codon_start=1
                     /product="gentamycin resistance protein"
                     /label=gentamycin resistance protein
                     /protein_id="ACS91924.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6579..6607)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"