LOCUS       Exported                6917 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLL1179, complete sequence.
ACCESSION   KY271342
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Lo J, Olson DG, Murphy SJ, Tian L, Hon S, Lanahan A, Guss AM, Lynd 
            LR.
  TITLE     Engineering electron metabolism to increase ethanol production in 
            Clostridium thermocellum
  JOURNAL   Metab. Eng. (2016) In press
  PUBMED    27989806
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Lo J, Olson DG, Murphy SJL., Tian L, Hon S, Lanahan AA, Guss AM, 
            Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2016) Thayer School of Engineering, Dartmouth 
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 6917)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Cloning vector pLL1179"
                     /mol_type="other DNA"
                     /db_xref="taxon:1930524"
     regulatory      1..209
                     /regulatory_class="promoter"
     CDS             210..1055
                     /codon_start=1
                     /gene="nfnA"
                     /product="NfnA"
                     /label=nfnA
                     /protein_id="APQ46211.1"
                     /translation="MFKIVKKKILNPQVKLMEISAPHVAKKAEPGQFIILRVNENGERI
                     PLTIADFDREKGTVTIIFQEVGKTTKLLGTLEEGDELLDFVGPLGKASHFENVKKAAVI
                     GGGLGTAIAYPQAKKLHSMGVEVHTIAGFRNKDLIILEDEMRAVSSKLFITTDDGSNGN
                     KGFVSDVLKKLIEEGNKYDLVVAIGPLIMMKVISELTRPYGIKTIVSMNPVMIDGTGMC
                     GGCRVTVGGEIKFACVDGPDFDGHLVDFDEAMRRQAMYKKEESLALEKHNCKLGVEKNA
                     "
     gene            210..1055
                     /gene="nfnA"
                     /label=nfnA
     CDS             1048..2442
                     /codon_start=1
                     /gene="nfnB"
                     /product="NfnB"
                     /label=nfnB
                     /protein_id="APQ46210.1"
                     /translation="MPNMSPKKVPMPEQDPNVRIKNFLEVALGYTEQMAMEEAQRCLNC
                     KHKPCVSGCPVNVKIPEFVQLIAQGKFEKAYNKIRETNNLPAICGRVCPQENQCEKFCV
                     RGIKGEPVAIGRLERFAADWHMKNGTTSYEKPEKNGKRVAVIGSGPASLTCASDLAKLG
                     YEVTIFEAFHVPGGVLMYGIPEFRLPKKLVQEEIETIKQLGVEIKTNMVIGKVYSIDEL
                     KAEGYDAIFIGSGAGLPSFMKIPGENLNGVYSANEFLTRINLMKAYEFPNCDTPVKVGK
                     NVAVVGGGNVAMDAARSAKRLGAENVYIVYRRSEAEMPARLEEIHHAKEEGILFKFLTN
                     PTRILGTDDGWVKGMECIEMELGEPDESGRRRPVPKPGSEHVIDVETVIIAIGQTPNPL
                     IASTTPGLATQKWGGIIVDENTGATNIEGVYAGGDAVTGAATVILAMGAGKKAAKAIDE
                     YLKNKK"
     gene            1048..2442
                     /gene="nfnB"
                     /label=nfnB
     CDS             2572..3222
                     /codon_start=1
                     /gene="cat"
                     /product="Cat"
                     /label=cat
                     /note="chloramphenicol aminotransferase"
                     /protein_id="APQ46212.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            2572..3222
                     /gene="cat"
                     /label=cat
     CDS             complement(3300..4160)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /note="ampicillin resistance protein"
                     /protein_id="APQ46213.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3300..4160)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(4161..4252)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4828..5652)
                     /direction=LEFT
     rep_origin      4852..5397
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      5687..5867
     CDS             5868..6872
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /note="replication initiator protein"
                     /protein_id="APQ46214.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            5868..6872
                     /gene="repB"
                     /label=repB