LOCUS       Exported                6563 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pLL102, complete sequence.
ACCESSION   JN639000
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Lei MG, Cue D, Alba J, Junecko J, Graham JW, Lee CY.
  TITLE     A single copy integration vector that integrates at an engineered 
            site on the Staphylococcus aureus chromosome
  JOURNAL   BMC Res Notes 5 (1), 5 (2012)
  PUBMED    22221385
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Lee CY.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2011) Microgiology and Immunology, University of 
            Arkansas for Medical Sciences, 4301 W. Markham St. slot 511, Little 
            Rock, AR 72205, USA
REFERENCE   3  (bases 1 to 6563)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Integration vector pLL102"
                     /lab_host="Staphylococcus aureus subsp. aureus NCTC 8325"
                     /strain="NCTC 8325"
                     /mol_type="other DNA"
                     /db_xref="taxon:1133351"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(712..2091)
                     /codon_start=1
                     /gene="tetK"
                     /product="TetK"
                     /label=tetK
                     /note="tetracycline efflux protein of pT181"
                     /protein_id="AEX86620.1"
                     /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHFN
                     TTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFF
                     ILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIA
                     HYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYN
                     WTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPY
                     MMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTI
                     AFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGT
                     GIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSEKQF
                     E"
     gene            complement(712..2091)
                     /gene="tetK"
                     /label=tetK
     misc_feature    2459..2476
                     /label=attP2 core sequence
                     /note="attP2 core sequence"
     CDS             complement(2819..3829)
                     /codon_start=1
                     /gene="aadA1"
                     /product="AadA1"
                     /label=aadA1
                     /note="aminoglycoside 3-adenylyltransferase"
                     /protein_id="AEX86621.1"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2819..3829)
                     /gene="aadA1"
                     /label=aadA1
     CDS             complement(2819..3610)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4862..5812)
                     /codon_start=1
                     /product="RepA"
                     /label=RepA
                     /protein_id="AEX86622.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5860..6082
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"