LOCUS       Exported                4448 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle DNA vaccine vector pLKV1, complete sequence.
ACCESSION   HM569775
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Tao L, Pavlova SI, Ji X, Jin L, Spear G.
  TITLE     A novel plasmid for delivering genes into mammalian cells with 
            noninvasive food and commensal lactic acid bacteria
  JOURNAL   Plasmid 65 (1), 8-14 (2010)
  PUBMED    20832422
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Tao L, Pavlova SI.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2010) Oral Biology, University of Illinois at 
            Chicago, 801 S. Paulina Street, Chicago, IL 60612, USA
REFERENCE   3  (bases 1 to 4448)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Shuttle DNA vaccine vector pLKV1"
                     /mol_type="other DNA"
                     /label=lactic acid bacteria-E. coli shuttle vector
                     /note="lactic acid bacteria-E. coli shuttle vector"
                     /db_xref="taxon:941988"
     CDS             complement(165..959)
                     /codon_start=1
                     /gene="aphA"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA
                     /note="kanamycin resistance protein"
                     /protein_id="ADU77108.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(165..959)
                     /gene="aphA"
                     /label=aphA
     enhancer        1591..1894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1895..2098
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2143..2161
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2308..2532
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             3566..3715
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A"
                     /label=repA
                     /note="derived from Lactobacillus plantarum"
                     /protein_id="ADU77109.1"
                     /translation="MDMERVKVGITLTEDTLARLEEICKEMGLSKSQALSMLVNKEYLE
                     KISR"
     gene            3566..3715
                     /gene="repA"
                     /label=repA
     CDS             3791..4381
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B"
                     /label=repB
                     /note="derived from Lactobacillus plantarum"
                     /protein_id="ADU77110.1"
                     /translation="MTKKKYEYEKGRDWTFIVYPESAPDNWRTVLDETHLLWIESPLHD
                     KDMNADGEIKKSHYHILLTFDGPVTEKQVIKLIDPLNTPLLKKLGCPRWFSVVIWHIWT
                     TLKNINTQRDEIVGHCGADVESYFELTKTSKMSVMKTIITYIYEIRLIIMLIFNDLYSN
                     ILMIGLTYRLTTTTLAINKMIDGMWLKKKNELR"
     gene            3791..4381
                     /gene="repB"
                     /label=repB