LOCUS       Exported                7284 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLionII-miniPOMC, complete sequence.
ACCESSION   JQ086322
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Hen G, Yosefi S, Shinder D, Or A, Mygdal S, Condiotti R, Galun E, 
            Bor A, Sela-Donenfeld D, Friedman-Einat M.
  TITLE     Gene Transfer to Chicks Using Lentiviral Vectors Administered via 
            the Embryonic Chorioallantoic Membrane
  JOURNAL   PLoS ONE 7 (5), E36531 (2012)
  PUBMED    22606269
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Chen G, Sela-Donenfeld D, Yosefi S, Condiotti R, Friedman-Einat M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2011) Animal Science, ARO, Volcani Center, 30 
            Derech Hamacabim, Beit-Dagan 50250, Israel
REFERENCE   3  (bases 1 to 7284)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Expression vector pLionII-miniPOMC"
                     /mol_type="other DNA"
                     /db_xref="taxon:1155072"
     rep_origin      167..786
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2874..3253
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      2954..3242
                     /regulatory_class="enhancer"
                     /note="CAG enhancer"
     promoter        3254..3457
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4352..4370
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        4352..4370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        4412..4791
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4413..5029
                     /regulatory_class="promoter"
                     /note="CMV immearly"
     promoter        4792..4995
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        5040..5058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5041..5058
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     CDS             5163..5387
                     /codon_start=1
                     /gene="POMC"
                     /product="truncated pro-opiomelanocortin preproprotein"
                     /label=POMC
                     /note="mini POMC; derived from Homo sapiens"
                     /protein_id="AFC36445.1"
                     /translation="MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLE
                     CIRACKPREGKRSYSMEHFRWGKPVGKKR"
     gene            5163..5387
                     /gene="POMC"
                     /label=POMC
     misc_feature    6193..6781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     regulatory      6200..6775
                     /regulatory_class="enhancer"
                     /note="WPRE regulatory element of woodchuck hepatitis 
                     virus"
     CDS             complement(6664..6675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6787..6803)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7064..7080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7088..7104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7112..7142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7157..7178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."