LOCUS       Exported                7674 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLHBA, complete sequence.
ACCESSION   AY234326
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Development of Plasmid Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Hausmann L, Toepfer R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2003) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 7674)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Binary vector pLHBA"
                     /mol_type="other DNA"
                     /db_xref="taxon:226211"
     misc_feature    2..103
                     /note="multiple cloning site from vector pBlueSfi BA in 
                     GenBank Accession Number AF327875"
     regulatory      9..18
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    104..706
                     /note="right border region from nopaline-type Ti plasmid 
                     C58 of Agrobacterium tumefaciens in GenBank Accession 
                     Number X07435"
     misc_difference 129
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    148..172
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    149..172
                     /label=right flanking T-DNA border
                     /note="right flanking T-DNA border"
     repeat_region   196..208
                     /label=repeat C
                     /note="repeat C"
     misc_difference 202
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     repeat_region   231..242
                     /label=repeat C
                     /note="repeat C"
     repeat_region   256..266
                     /label=repeat B
                     /note="repeat B"
     repeat_region   283..293
                     /label=repeat B
                     /note="repeat B"
     misc_difference 288
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_difference 299
                     /replace="a"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    complement(316..706)
                     /note="3 sequence of the ornithine cyclodeaminase gene 
                     ocd; non-functional"
     misc_difference 492
                     /replace="t"
                     /label=compared to GenBank Accession Number X07435
                     /note="compared to GenBank Accession Number X07435"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     rep_origin      707..1871
                     /note="ColE1 origin of replication sequence of pBR322; 
                     similar to GenBank Accession Number J01749; serves as 
                     origin of replication in Escherichia coli host"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     rep_origin      817..1405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 964
                     /replace="c"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_difference 1503..1504
                     /replace="gta"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    1590..1730
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    1683
                     /label=nic/bom site
                     /note="nic/bom site"
     rep_origin      1887..5658
                     /note="origin of replication and partitioning region from 
                     plasmid pVS1 of Pseudomonas; similar to GenBank Accession 
                     Number U10487; serves as origin of replication in 
                     Agrobacterium tumefaciens host"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     regulatory      2018..2023
                     /regulatory_class="minus_35_signal"
     regulatory      2044..2049
                     /regulatory_class="minus_10_signal"
     regulatory      2066..2070
                     /regulatory_class="ribosome_binding_site"
     CDS             2080..2766
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid 
                     pVS1; similar to Tn3 resolvase in GenBank Accession Number 
                     V00613, Tn917 resolvase in GenBank Accession Number M11180,
                     and RK2 ParA in GenBank Accession Number L27758"
                     /protein_id="AAO85341.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     misc_difference 2499
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI 
                     restriction site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     stem_loop       2910..2942
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_difference 2918
                     /replace="g"
                     /note="nucleotide changed to conserve palindromic sequence 
                     following SfiI restriction site-destruction"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_difference 2933
                     /replace="c"
                     /note="original nucleotide changed to destroy two SfiI 
                     restriction sites"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     regulatory      3000..3005
                     /regulatory_class="minus_35_signal"
     regulatory      3021..3026
                     /regulatory_class="minus_10_signal"
     regulatory      3054..3061
                     /regulatory_class="ribosome_binding_site"
     CDS             3065..3694
                     /codon_start=1
                     /product="ParA"
                     /label=ParA
                     /note="partitioning protein ParA encoded by parA of plasmid
                     pVS1; similar to F plasmid SopA in GenBank Accession Number
                     X04619, RK2 IncC in GenBank Accession Number L27758, and P1
                     ParA in GenBank Accession Number X02954"
                     /protein_id="AAO85342.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3961..3973
                     /note="identical to the korB operator binding site of 
                     plasmid RK2 in GenBank Accession Number L27758.1"
     CDS             4021..5196
                     /codon_start=1
                     /product="ParB"
                     /label=ParB
                     /note="partitioning protein ParB encoded by parB of plasmid
                     pVS1; similar to F plasmid SopB in GenBank Accession Number
                     X04619, RK2 KorB in GenBank Accession Number L27758, and P1
                     ParB in GenBank Accession Number X02954"
                     /protein_id="AAO85343.1"
                     /translation="MPHVWRNGRLARRKRLGCLPALQWHWNPQARGIGVSGRKPSGPVQ
                     IGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKP
                     PKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGP
                     AGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKD
                     DLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELAD
                     WMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSA
                     GEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
     CDS             4123..5196
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     misc_difference 4127
                     /label=compared to GenBank Accession Number U10487
                     /note="compared to GenBank Accession Number U10487"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_difference 4200
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI 
                     restriction site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_difference 4521
                     /replace="c"
                     /note="original nucleotide changed to destroy a SfiI 
                     restriction site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     repeat_region   5188..5202
                     /label=repeat D
                     /note="repeat D"
     stem_loop       5210..5232
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_feature    5239..5658
                     /note="parC region; contains putative cis-acting sites for 
                     replication, partition and incompatibility"
     rep_origin      5262..5456
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     protein_bind    5262..5270
                     /label=DnaA binding site
                     /bound_moiety="DnaA"
                     /note="putative DnaA box"
     repeat_region   5273..5287
                     /label=repeat D
                     /note="repeat D"
     repeat_region   5294..5308
                     /label=repeat D
                     /note="repeat D"
     repeat_region   5315..5329
                     /label=repeat D
                     /note="repeat D"
     repeat_region   5336..5350
                     /label=repeat D
                     /note="repeat D"
     misc_feature    5359..5437
                     /label=AT-rich region
                     /note="AT-rich region"
     protein_bind    5449..5457
                     /label=DnaA binding site
                     /bound_moiety="DnaA"
                     /note="putative DnaA box"
     repeat_region   5553..5566
                     /label=repeat E
                     /rpt_unit_range=5553 .. 5559
                     /note="repeat E"
     misc_feature    5666..7070
                     /label=similar to GenBank Accession Number M35007
                     /note="similar to GenBank Accession Number M35007"
     regulatory      5797..5802
                     /regulatory_class="minus_35_signal"
     regulatory      5820..5825
                     /regulatory_class="minus_10_signal"
     CDS             5842..6852
                     /codon_start=1
                     /gene="aadA"
                     /product="streptomycin/spectinomycin adenyltransferase"
                     /label=aadA
                     /note="confers resistance to streptomycin and 
                     spectinomycin"
                     /protein_id="AAO85344.1"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            5842..6852
                     /gene="aadA"
                     /label=aadA
                     /note="different start codons are described in the 
                     literature"
     CDS             6061..6852
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     stem_loop       6855..6912
                     /note="palindromic sequence; putative termination signal"
     misc_feature    7071..7672
                     /note="left border region from octopine-type Ti plasmid of 
                     Agrobacterium tumefaciens; similar to GenBank Accession 
                     Number X00493"
     misc_difference 7089..7090
                     /replace="ggtacc"
                     /note="compared to GenBank Accession Number X00493; removed
                     to destroy a KpnI restriction site"
     misc_feature    7372..7396
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    7373..7396
                     /label=left flanking T-DNA border
                     /note="left flanking T-DNA border"