LOCUS       Exported                7493 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLG338, complete sequence.
ACCESSION   KM604642
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Mitarai N, Jensen MH, Semsey S.
  TITLE     Coupled positive and negative feedbacks produce diverse gene 
            expression patterns in colonies
  JOURNAL   MBio 6 (2), e00059-15 (2015)
  PUBMED    25852158
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Semsey S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2014) Niels Bohr Institute, University of 
            Copenhagen, Blegdamsvej 17, Copenhagen 2100, Denmark
REFERENCE   3  (bases 1 to 7493)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Cloning vector pLG338"
                     /mol_type="other DNA"
                     /db_xref="taxon:1622173"
     mobile_element  328..711
                     /mobile_element_type="transposon:Tn903"
     CDS             1729..2106
                     /codon_start=1
                     /gene="act"
                     /product="acetyltransferase"
                     /label=act
                     /protein_id="AJR21177.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSGQ
                     LHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGGRI
                     NDHIVNYYLHGESLSKLASGI"
     gene            1729..2106
                     /gene="act"
                     /label=act
     promoter        2760..2788
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             2836..4026
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline resistance protein"
                     /label=tet
                     /protein_id="AJR21174.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            2836..4026
                     /gene="tet"
                     /label=tet
     rep_origin      4391..4613
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4661..5611
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiation protein"
                     /label=rep
                     /protein_id="AJR21175.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELITRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            4661..5611
                     /gene="rep"
                     /label=rep
     mobile_element  6479..6869
                     /mobile_element_type="transposon:Tn903"
     CDS             6945..267
                     /codon_start=1
                     /gene="aphA1"
                     /allele="Tn903"
                     /product="aminoglycoside 3-phosphotransferase"
                     /label=aphA1
                     /protein_id="AJR21176.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            6945..267
                     /gene="aphA1"
                     /allele="Tn903"
                     /label=aphA1