LOCUS       Exported                6392 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plastid transformation vector pLEVAtL-R1, complete sequence.
ACCESSION   KP635964
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Whitney SM, Birch R, Kelso C, Beck JL, Kapralov MV.
  TITLE     Improving recombinant Rubisco biogenesis, plant photosynthesis and 
            growth by coexpressing its ancillary RAF1 chaperone
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (11), 3564-3569 (2015)
  PUBMED    25733857
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Whitney SM, Birch R, Kelso C, Beck JL, Kapralov MV.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2015) Research School of Biology, The Australian 
            National University, Acton, Canberra, ACT 2601, Australia
REFERENCE   3  (bases 1 to 6392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Plastid transformation vector pLEVAtL-R1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1627830"
     misc_feature    1..1106
                     /note="tobacco plastome homologous recombination sequence; 
                     similar to GenBank Accession Number Z00044"
     CDS             1107..2546
                     /codon_start=1
                     /gene="rbcL"
                     /product="ribulose-1,5-bisphosphate carboxylase/oxygenase 
                     large subunit"
                     /label=rbcL
                     /note="derived from Arabidopsis thaliana"
                     /protein_id="AJS15038.1"
                     /translation="MSPQTETKASVGFKAGVKEYKLTYYTPEYETKDTDILAAFRVTPQ
                     PGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYHIEPVPGEETQFIAYVAYP
                     LDLFEEGSVTNMFTSIVGNVFGFKALAALRLEDLRIPPAYTKTFQGPPHGIQVERDKLN
                     KYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCA
                     EAIYKSQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLS
                     HYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRLSGGDHIHAGTVVGKLEGDRE
                     STLGFVDLLRDDYVEKDRSRGIFFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSV
                     LQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAVEGNEIIREACKWSPELAAA
                     CEVWKEITFNFPTIDKLDGQE"
     gene            1107..2546
                     /gene="rbcL"
                     /label=rbcL
     CDS             2586..3959
                     /codon_start=1
                     /gene="raf1"
                     /product="rubisco accumulation factor 1"
                     /label=raf1
                     /note="synthetic raf1 gene derived from Arabidopsis 
                     thaliana; codon modified to match rbcL"
                     /protein_id="AJS15039.1"
                     /translation="MAPLKSLISSPFTQSTTHGLFTNPITRPVNPLPRTVSFTVTASMI
                     PKRSSANMIPKNPPARQQLYQPFRPPSSPIPTQFRSLDSAGKIEILAGRMALWFEYAPL
                     ISSLYTDGFTPPTIEELTGISSIEQNRLIVGAQVRDSILQSIHEPELISAFDTGGAELL
                     YEIRLLSTTQRVAAATFIIDRNIDSKGAQDLARAIKDYPNRRGDVGWLDFDYNLPGDCL
                     SFLYYRQSRENKNPSDQRTSMLLQALGVAESEKAKNRLNTELYGDKEAEKEKEKKKKEE
                     EVKAIRIPVVRLKFGEVAEATSVVVLPVCKAEEGEKKILEAPMEIIAGGDFKVVEAEKG
                     WKRWVVLPSWNPVAAIGKGGVAVSFRDDRKVLPWDGKEEPLLVVADRVRNVVEADDGYY
                     LVVAENGLKLEKGSDLKAREVKESLGMVVLVVRPPREDDDDWQTSHQNWDLEHHHHHH"
     gene            2586..3959
                     /gene="raf1"
                     /label=raf1
     CDS             3939..3956
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    3967..4227
                     /note="tobacco plastome homologous recombination sequence; 
                     similar to GenBank Accession Number Z00044"
     CDS             4247..5038
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenine transferase"
                     /label=aadA
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /protein_id="AJS15040.1"
                     /translation="MAEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVLEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4247..5038
                     /gene="aadA"
                     /label=aadA
     3UTR           5051..5199
                     /gene="rps16"
     gene            5051..5199
                     /gene="rps16"
                     /label=rps16
     misc_feature    5210..6392
                     /note="tobacco plastome homologous recombination sequence; 
                     similar to GenBank Accession Number Z00044"