LOCUS       Exported               11925 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pLEIItd+KRdeltaORF, complete sequence.
ACCESSION   AY303237
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Staddon JH, Bryan EM, Manias DA, Dunny GM.
  TITLE     Conserved target for group II intron insertion in relaxase genes of 
            conjugative elements of gram-positive bacteria
  JOURNAL   J. Bacteriol. 186 (8), 2393-2401 (2004)
  PUBMED    15060042
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   Staddon JH.
  TITLE     Ll.ltrB group II intron donor plasmid with a large deletion in the 
            intron encoded protein ORF, ltrA
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11925)
  AUTHORS   Staddon JH.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2003) Microbiology, University of Minnesota, MMC 
            196, 420 Delaware Street S.E., Minneapolis, MN 55455, USA
REFERENCE   4  (bases 1 to 11925)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shuttle vector pLEIItd+KRdeltaORF"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:234876"
     exon            1623..1802
                     /number=1
                     /note="ltrBE1; partial exon"
     intron          1803..4225
                     /note="Ll.ltrBdeltaORF group II intron with APHIII and 
                     tddelta(1-3)"
     CDS             2376..2585
                     /codon_start=1
                     /product="LtrAdeltaORF"
                     /label=LtrAdeltaORF
                     /note="maturase with internal deletion"
                     /protein_id="AAP73797.1"
                     /translation="MKPTMAILERISKNSQENIDEVFTRLYRYLLRPDIYYVATRWEMA
                     MIAKQRKTLVVCFHCHRHVIHKHK"
     exon            2594..2605
                     /number=1
                     /note="derived from T4 phage thymidylate synthase found in 
                     GenBank Accession Number M12742"
     intron          2606..2998
                     /note="tddelta(1-3) group I intron"
     exon            2999..3010
                     /number=2
                     /note="derived from T4 phage thymidylate synthase found in 
                     GenBank Accession Number M12742; partial exon"
     CDS             3255..4049
                     /codon_start=1
                     /product="APH-III"
                     /label=APH-III
                     /note="3,5aminoglycoside phosphotransferase; kanamycin 
                     resistance; from enterococcal plasmid pJH1 found in GenBank
                     Accession Number V01547"
                     /protein_id="AAP73802.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     exon            4226..4316
                     /number=2
                     /note="ltrBE2; partial exon"
     CDS             complement(5166..6656)
                     /codon_start=1
                     /product="replication protein E"
                     /label=replication protein E
                     /note="pIL253 replication in gram positive bacteria; 
                     derived from GenBank Accession Number AF041239"
                     /protein_id="AAP73798.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK
                     ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     CDS             complement(6699..6998)
                     /codon_start=1
                     /product="replication protein D"
                     /label=replication protein D
                     /note="pIL253 replication in gram positive bacteria; 
                     derived from GenBank Accession Number AF041239"
                     /protein_id="AAP73799.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKSD
                     KYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     CDS             complement(7893..7904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8339..8989
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /note="functions in gram positive bacteria; derived from 
                     pC194 found in GenBank Accession Number V01277"
                     /protein_id="AAP73800.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             complement(10617..11276)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /note="functions in gram negative bacteria; derived from 
                     plasmid pACYC184 found in GenBank Accession Number X06403"
                     /protein_id="AAP73801.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(11277..11379)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"