LOCUS       Exported                5958 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLECL, complete sequence.
ACCESSION   JQ825240
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 5958)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Cloning vector pLECL"
                     /mol_type="other DNA"
                     /note="cloning vector for C-terminal mCFP-1xMYC taging in 
                     fission yeast"
                     /db_xref="taxon:1276085"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    302..1012
                     /label=mCFP
                     /note="mCFP"
     misc_feature    1013..1042
                     /label=1xMYC tag
                     /note="1xMYC tag"
     CDS             1013..1042
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      1052..1544
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae Leu2 terminator"
     regulatory      1551..2196
                     /regulatory_class="promoter"
                     /note="Saccharomyces cerevisiae Leu2 promoter"
     promoter        1791..2196
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2197..3291
                     /codon_start=1
                     /product="Leu2"
                     /label=Leu2
                     /note="Saccharomyces cerevisiae 3-isopropylmalate 
                     dehydrogenase"
                     /protein_id="AGB50981.1"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     regulatory      3292..3768
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae Leu2 terminator"
     promoter        complement(3847..3865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4108..4727
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4882..5742)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="beta-lactamase"
                     /protein_id="AGB50982.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5743..5847)
                     /gene="bla"
                     /label=AmpR promoter