LOCUS       Exported                4105 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLDR9, complete sequence.
ACCESSION   AF271663
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Wiencis AM, Keagle P, Andersen J, Wotanis J, Newcombe R, August PR.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2000) Molecular Genomics, Aventis Pharmaceuticals,
            26 Landsdowne Street, Cambridge, MA 02139, USA
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Cloning vector pLDR9"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:130501"
     CDS             216..1031
                     /codon_start=1
                     /product="aminoglycoside 3 phosphotransferase"
                     /function="confers kanamycin resistance"
                     /label=aminoglycoside 3 phosphotransferase
                     /protein_id="AAF82363.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        1423..1527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1528..2388
                     /codon_start=1
                     /product="beta-lactamase"
                     /EC_number="3.5.2.6"
                     /label=beta-lactamase
                     /note="derived from pMMB66EH"
                     /protein_id="AAF82362.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(1992..2258)
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="hypothetical 9.2 kD protein"
                     /protein_id="AAF82364.1"
                     /translation="MIPRDPRSPAPDLSAINQPAGRAERRSGPATLSASIQSINCCREA
                     RVSSSPVNSLRNVVAIATGIVVSRSSFGMASFSSGSQRSRRVT"
     misc_feature    2612..2859
                     /label=attP
                     /note="attP"
     protein_bind    2622..2853
                     /gene="attP"
                     /label=-lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     rep_origin      complement(3276..3864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"