LOCUS       Exported                4604 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLD7-rrnP-MCS DNA, complete sequence.
ACCESSION   AB375764
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Hasunuma T, Miyazawa SI, Yoshimura S, Shinzaki Y, Tomizawa KI, 
            Shindo K, Choi SK, Misawa N, Miyake C.
  TITLE     Biosynthesis of astaxanthin in tobacco leaves by transplastomic 
            engineering
  JOURNAL   Plant J. (2008) In press
  PUBMED    18494855
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Hasunuma T, Miyake C.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2008) Contact:Tomohisa Hasunuma Research Institute
            of Innovative Technology for the Earth, Plant Research Group; 9-2 
            Kizugawadai, Kizugawa-shi, Kyoto 619-0292, Japan
REFERENCE   3  (bases 1 to 4604)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Expression vector pLD7-rrnP-MCS"
                     /mol_type="other DNA"
                     /db_xref="taxon:497690"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2237..2353
                     /regulatory_class="promoter"
                     /note="tobacco rrn promoter"
     CDS             2372..3166
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside 3-adenylyltransferase"
                     /label=aadA
                     /protein_id="BAG06710.1"
                     /translation="MAREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPA
                     KRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDL
                     FEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQ
                     PVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2372..3166
                     /gene="aadA"
                     /label=aadA
     regulatory      3592..3708
                     /regulatory_class="promoter"
                     /note="tobacco rrn promoter"
     promoter        complement(3961..3979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3989..4005)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4147..4602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"