LOCUS       Exported                5590 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLCPVRGNR104 DNA, complete sequence.
ACCESSION   AB038602
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Hashinaka K.
  TITLE     Synthetic Autonomous Vectors Based on Palindromic Sequences of 
            Parvovirus B19
  JOURNAL   Published Only in Database (2001)
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Hashinaka K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2000) Kazuya Hashinaka, Miyazaki Medical College, 
            Department of Biochemistry; 5200 Kihara, Kiyotake, Miyazaki 
            889-1692, Japan (E-mail:hasinaka@post1.miyazaki-med.ac.jp, 
            Tel:81-985-85-0985, Fax:81-985-85-2401)
REFERENCE   3  (bases 1 to 5590)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Cloning vector pLCPVRGNR104"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:117920"
     source          12..485
                     /organism="Human parvovirus B19"
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
     source          3369..3846
                     /organism="Human parvovirus B19"
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
     repeat_region   15..397
     polyA_signal    514..635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    863..1087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1115..2635)
                     /codon_start=1
                     /gene="rsGFP-neoR"
                     /product="red-shift green fluorescent protein-fused 
                     neomycin phosphotransferase"
                     /label=rsGFP-neoR
                     /protein_id="BAB32740.1"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYNGAGAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL
                     SAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVP
                     GQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVAD
                     RYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(1115..2635)
                     /gene="rsGFP-neoR"
                     /label=rsGFP-neoR
     CDS             complement(1115..1903)
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="EQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVL
                     FVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSH
                     LAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGL
                     APAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     repeat_region   3455..3837
     rep_origin      3930..4518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4579..4600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4615..4645
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4653..4669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4677..4693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4834..5493)
                     /codon_start=1
                     /gene="CmR"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /protein_id="BAB32741.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVHLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHTEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(4834..5493)
                     /gene="CmR"
                     /label=CmR