LOCUS       Exported               10684 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plant binary vector pLC41 DNA, complete sequence.
ACCESSION   LC215698
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Ishida Y, Hiei Y, Komari T.
  TITLE     High Efficiency Wheat Transformation Mediated by Agrobacterium 
            tumefaciens
  JOURNAL   Advances in Wheat Genetics: From Genome to Field: Proceedings of the
            12th (International Wheat Genetics Symposium), 167-173 (2015)
REFERENCE   2  (bases 1 to 10684)
  AUTHORS   Tsunashima M, Komori T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2017) Contact:Masako Tsunashima JAPAN TOBACCO 
            INC., Plant Innovation Center; 700 Higashibara, Iwata, Shizuoka 
            438-0802, Japan
REFERENCE   3  (bases 1 to 10684)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Plant binary vector pLC41"
                     /mol_type="other DNA"
                     /db_xref="taxon:1957018"
     misc_feature    104..128
                     /label=Left Border of T-DNA
                     /note="Left Border of T-DNA"
     misc_feature    711..1109
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    complement(915..926)
                     /label=cos
                     /note="cos"
     CDS             1778..2872
                     /codon_start=1
                     /gene="IncC1"
                     /product="IncC1"
                     /label=IncC1
                     /protein_id="BAW98481.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            1778..2872
                     /gene="IncC1"
                     /label=IncC1
     CDS             1791..2096
                     /codon_start=1
                     /gene="korA"
                     /product="korA"
                     /label=korA
                     /protein_id="BAW98482.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            1791..2096
                     /gene="korA"
                     /label=korA
     CDS             2093..2872
                     /codon_start=1
                     /gene="IncC2"
                     /product="IncC2"
                     /label=IncC2
                     /protein_id="BAW98483.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            2093..2872
                     /gene="IncC2"
                     /label=IncC2
     CDS             2869..3945
                     /codon_start=1
                     /gene="korB"
                     /product="korB"
                     /label=korB
                     /protein_id="BAW98484.1"
                     /translation="MTAAQAKTTKKNTAAAAQEAAGAAQPSGLGLDSIGDLSSLLDAPA
                     ASQGGSGPIELDLDLIDEDPHQPRTADNPGFSPESIAEIGATIKERGVKSPISVRENQE
                     QPGRYIINHGARRYRGSKWAGKKSIPAFIDNDYNEADQVIENLQRNELTPREIADFIGR
                     ELAKGKKKGDIAKEIGKSPAFITQHVTLLDLPEKIADAFNTGRVRDVTVVNELVTAFKK
                     RPEEVEAWLDDDTQEITRGTVKLLREFLDEKGRDPNTVDAFNGQTDAERDAEAGDGQDG
                     EDGDQDGKDAKEKGAKEPDPDKLKKAIVQVEHDERPARLILNRRPPAEGYAWLKYEDDG
                     QEFEANLADVKLVALIEG"
     gene            2869..3945
                     /gene="korB"
                     /label=korB
     oriT            complement(4181..5002)
                     /direction=LEFT
                     /note="oriT"
     oriT            4351..4460
                     /note="incP origin of transfer"
     CDS             4493..4864
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             complement(5148..5459)
                     /codon_start=1
                     /gene="trbA"
                     /product="trbA"
                     /label=trbA
                     /protein_id="BAW98485.1"
                     /translation="MTKHELSERAGVSISFLSDLTNGKANPSLKVMEAIADALETPLPL
                     LLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAFIVKKWGDDTRKKLRGRL"
     gene            complement(5148..5459)
                     /gene="trbA"
                     /label=trbA
     CDS             5634..5984
                     /codon_start=1
                     /gene="ssb"
                     /product="ssb"
                     /label=ssb
                     /protein_id="BAW98486.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            5634..5984
                     /gene="ssb"
                     /label=ssb
     CDS             6033..7181
                     /codon_start=1
                     /gene="trfA2"
                     /product="trfA2"
                     /label=trfA2
                     /protein_id="BAW98487.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            6033..7181
                     /gene="trfA2"
                     /label=trfA2
     CDS             6324..7181
                     /codon_start=1
                     /gene="trfA1"
                     /product="trfA1"
                     /label=trfA1
                     /protein_id="BAW98488.1"
                     /translation="MTTKKRKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAP
                     WPDTMRAMPNDTARSALFTTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELV
                     WQQVLEYAKRTPIGEPITFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRV
                     GHLESVSLLHRFRVLDRGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRM
                     FDYFSSHREPYPLKLETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLV
                     HCKR"
     gene            6324..7181
                     /gene="trfA1"
                     /label=trfA1
     CDS             complement(8299..9093)
                     /codon_start=1
                     /gene="NptIII"
                     /product="NptIII"
                     /label=NptIII
                     /protein_id="BAW98489.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(8299..9093)
                     /gene="NptIII"
                     /label=NptIII
     rep_origin      9433..10144
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      complement(9451..10068)
                     /direction=LEFT
                     /label=oriV
                     /note="oriV"
     misc_feature    10508..10532
                     /label=Right Border of T-DNA
                     /note="Right Border of T-DNA"