LOCUS       Exported                8030 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pKZ2, complete sequence.
ACCESSION   KY615709
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of 
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2017) College of Animal Science & Technology, 
            Northwest A&F University, 22 St. Xinong, Yangling, Shaanxi 712100, 
            China
REFERENCE   3  (bases 1 to 8030)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Shuttle vector pKZ2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2022389"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     of replication"
     CDS             complement(953..1813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /protein_id="ASN64774.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(953..1813)
                     /gene="bla"
                     /label=bla
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2392..2408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3456..4055
                     /codon_start=1
                     /product="RepC"
                     /label=RepC
                     /note="repC; temperature sensitive replicon"
                     /protein_id="ASN64775.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             complement(4388..5011)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=tetR
                     /protein_id="ASN64776.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(4388..5011)
                     /gene="tetR"
                     /label=tetR
     protein_bind    5036..5054
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5121..5139
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     misc_feature    complement(5144..5716)
                     /label=antisense-secY
                     /note="antisense-secY"
     CDS             6410..7060
                     /codon_start=1
                     /product="chloramphenicol resistance protein"
                     /label=chloramphenicol resistance protein
                     /note="cat(+)"
                     /protein_id="ASN64777.1"
                     /translation="MKFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     misc_feature    7240..7283
                     /label=MCS
                     /note="MCS"
     CDS             7532..7930
                     /codon_start=1
                     /product="bleomycin resistance protein"
                     /label=bleomycin resistance protein
                     /note="bleoR"
                     /protein_id="ASN64778.1"
                     /translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI
                     HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL
                     KDQWWDERDFAVIDPDNNLISFFQQIKS"