LOCUS       Exported                9913 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKZ2-attS-C2, complete sequence.
ACCESSION   KY615711
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of 
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2017) College of Animal Science & Technology, 
            Northwest A&F University, 22 St. Xinong, Yangling, Shaanxi 712100, 
            China
REFERENCE   3  (bases 1 to 9913)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Cloning vector pKZ2-attS-C2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2022393"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(953..1813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ASN64768.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(953..1813)
                     /gene="bla"
                     /label=bla
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2392..2408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3456..4055
                     /codon_start=1
                     /gene="repC"
                     /product="temperature sensitive replication protein RepC"
                     /label=repC
                     /protein_id="ASN64769.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     gene            3456..4055
                     /gene="repC"
                     /label=repC
     CDS             complement(4388..5011)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=tetR
                     /protein_id="ASN64770.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(4388..5011)
                     /gene="tetR"
                     /label=tetR
     protein_bind    5036..5054
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5121..5139
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     misc_feature    complement(5144..5716)
                     /label=antisense-secY
                     /note="antisense-secY"
     CDS             6410..7060
                     /codon_start=1
                     /gene="cat(+)"
                     /product="chloramphenicol resistance protein"
                     /label=cat(+)
                     /protein_id="ASN64771.1"
                     /translation="MKFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            6410..7060
                     /gene="cat(+)"
                     /label=cat(+)
     regulatory      7272..7370
                     /regulatory_class="promoter"
                     /note="PmecA"
     CDS             7368..9128
                     /codon_start=1
                     /gene="ccrC2"
                     /product="cassette chromosome recombinase CcrC2"
                     /label=ccrC2
                     /note="N-terminal attSCC tag"
                     /protein_id="ASN64772.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     gene            7368..9128
                     /gene="ccrC2"
                     /label=ccrC2
     repeat_region   7382..7420
                     /gene="ccrC2"
                     /label=attSCC
                     /note="attSCC"
     repeat_region   7399..7417
                     /gene="ccrC2"
                     /label=Direct repeat
                     /note="Direct repeat"
     CDS             9415..9813
                     /codon_start=1
                     /gene="bleoR"
                     /product="bleomycin resistance protein"
                     /label=bleoR
                     /protein_id="ASN64773.1"
                     /translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI
                     HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL
                     KDQWWDERDFAVIDPDNNLISFFQQIKS"
     gene            9415..9813
                     /gene="bleoR"
                     /label=bleoR