LOCUS       Exported               14271 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  His-3 integration vector pKYAM026, complete sequence.
ACCESSION   AY189825
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Construction of strains for rapid homokaryon purification after 
            integration of constructs at the histidine-3 (his-3) locus of 
            Neurospora crassa
  JOURNAL   Curr. Genet. 43 (1), 17-23 (2003)
  PUBMED    12684841
REFERENCE   2  (bases 1 to 14271)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2002) Biology, Texas A&M University, BSBW 415, 
            College Station, TX 77843-3258, USA
REFERENCE   3  (bases 1 to 14271)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="his-3 integration vector pKYAM026"
                     /focus
                     /mol_type="genomic DNA"
                     /db_xref="taxon:220723"
     source          2584..5465
                     /organism="Cloning vector pGEM-9Zf(-)"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:90118"
     misc_feature    complement(1..2583)
                     /label=benomyl resistant marker
                     /note="benomyl resistant marker"
     promoter        complement(2590..2608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2638..2654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2662..2678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2686..2716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2731..2752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3040..3628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3799..4659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4660..4764)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4791..5246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5387..5403
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5432..5450
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    5466..14271
                     /label=his-3 HindIII fragment
                     /note="his-3 HindIII fragment"
     CDS             complement(10385..10396)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    10661..10713
                     /label=multi-cloning sites
                     /note="multi-cloning sites"