LOCUS       Exported                5368 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression and selection vector pKS336, complete sequence.
ACCESSION   AF403737
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Saijo M, Qing T, Niikura M, Maeda A, Ikegami T, Sakai K, Prehaud C, 
            Kurane I, Morikawa S.
  TITLE     Immunofluorescence technique using HeLa cells expressing recombinant
            nucleoprotein for detection of immunoglobulin G antibodies to 
            Crimean-Congo hemorrhagic fever virus
  JOURNAL   J. Clin. Microbiol. 40 (2), 372-375 (2002)
  PUBMED    11825944
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Sakai K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2001) AIDS Research Center, National Institute of 
            Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, 
            Japan
REFERENCE   3  (bases 1 to 5368)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Expression and selection vector pKS336"
                     /plasmid="pKS336"
                     /mol_type="other DNA"
                     /db_xref="taxon:169293"
     regulatory      1..203
                     /regulatory_class="promoter"
                     /note="from human elongation factor-1-alpha"
     promoter        25..236
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     exon            204..236
                     /number=1
                     /note="from human elongation factor-1-alpha"
     intron          237..1183
                     /number=A
                     /note="from human elongation factor-1-alpha"
     intron          980..1183
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     exon            1184..1192
                     /number=2
                     /note="from partial human elongation factor-1-alpha exon"
     misc_feature    1194..1243
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     polyA_signal    1239..1373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1313..1318)
                     /regulatory_class="polyA_signal_sequence"
                     /note="from SV40 early mRNA"
     regulatory      complement(1342..1347)
                     /regulatory_class="polyA_signal_sequence"
                     /note="from SV40 early mRNA"
     regulatory      1362..1367
                     /regulatory_class="polyA_signal_sequence"
                     /note="from SV40 late mRNA"
     CDS             complement(1798..1818)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1948..2013
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     intron          complement(1948..2012)
                     /note="from SV40 small t antigen"
     CDS             complement(2259..2681)
                     /codon_start=1
                     /gene="bsr"
                     /product="blasticidin-S deaminase"
                     /label=bsr
                     /protein_id="AAK95216.1"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     gene            complement(2259..2681)
                     /gene="bsr"
                     /label=bsr
     regulatory      complement(2724..3067)
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter region"
     promoter        complement(2729..3058)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2743..2878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    3209..3349
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3535..4123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3536
                     /label=pBR322
                     /note="pBR322"
     CDS             complement(4294..5154)
                     /codon_start=1
                     /gene="amp"
                     /product="beta-lactamase"
                     /label=amp
                     /protein_id="AAK95217.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4294..5154)
                     /gene="amp"
                     /label=amp
     promoter        complement(5155..5259)
                     /gene="bla"
                     /label=AmpR promoter