LOCUS       Exported                9266 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKS300IC DNA, complete sequence.
ACCESSION   LC127362
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Fujiyama A.
  TITLE     Cloning vector pKS300IC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Fujiyama A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2016) Contact:Asao Fujiyama National Instetute of 
            Genetics, Center for Information Biology; 1111 Yata, Mishima, 
            Shizuoka 411-8540, Japan URL :http://www.nig.ac.jp/
REFERENCE   3  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Cloning vector pKS300IC"
                     /mol_type="other DNA"
                     /db_xref="taxon:1807768"
     primer_bind     complement(86..108)
                     /label=pKS300IC_Rev
                     /note="pKS300IC_Rev"
     promoter        160..264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             265..1125
                     /codon_start=1
                     /gene="blaTEM-1"
                     /product="beta-lactamase"
                     /function="Beta-lactamase class A"
                     /label=blaTEM-1
                     /note="Beta-lactamase enzyme family; pfam13354; 
                     beta-lactamase TEM; Provisional; beta-lactamase 
                     [Escherichia coli]; clinical isolate; gene_1; identified by
                     MetaGeneAnnotator; putative"
                     /protein_id="BAU61621.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            265..1125
                     /gene="blaTEM-1"
                     /label=blaTEM-1
     misc_feature    1610..2083
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2156..3127)
                     /codon_start=1
                     /gene="sopB"
                     /product="plasmid partitioning protein"
                     /function="Predicted transcriptional regulators"
                     /label=sopB
                     /note="ParB-like nuclease domain; smart00470; clinical 
                     isolate; gene_2; identified by MetaGeneAnnotator; putative;
                     plasmid partitioning protein [Escherichia coli]; 
                     plasmid-partitioning protein; Provisional"
                     /protein_id="BAU61622.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(2156..3127)
                     /gene="sopB"
                     /label=sopB
     CDS             complement(3127..4302)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             complement(3127..4293)
                     /codon_start=1
                     /product="plasmid-partitioning protein SopA"
                     /function="ATPases involved in chromosome partitioning"
                     /label=plasmid-partitioning protein SopA
                     /note="DNA binding residues [nucleotide binding]; Magnesium
                     ion binding site [ion binding]; MerR family regulatory 
                     protein; pfam00376; P-loop; ParA and ParB of Caulobacter 
                     crescentus belong to a conserved family of bacterial 
                     proteins implicated in chromosome segregation. ParB binds 
                     to DNA sequences adjacent to the origin of replication and 
                     localizes to opposite cell poles shortly following the...; 
                     cd02042; enterohemorrhagic; EHEC; sorbitol-fermenting; 
                     gene_3; identified by MetaGeneAnnotator; putative; 
                     plasmid-partitioning protein SopA [Escherichia coli]; 
                     plasmid-partitioning protein SopA; Provisional; similar to 
                     SopA protein of Escherichia coli plasmid F and pO157; 
                     plasmid partitioning protein"
                     /protein_id="BAU61623.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGEA
                     ADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAEDV
                     FPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDLHIH
                     AEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTDPHL
                     MLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFFDML
                     RDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDEVGK
                     GQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    4628..4878
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4881..5636)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein of the FIA 
                     replicon"
                     /function="Protein involved in initiation of plasmid 
                     replication"
                     /label=repE
                     /note="clinical isolate; gene_4; identified by 
                     MetaGeneAnnotator; putative; replication initiation protein
                     of the FIA replicon [Escherichia coli]; replication 
                     protein; Provisional"
                     /protein_id="BAU61624.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(4881..5636)
                     /gene="repE"
                     /label=repE
     rep_origin      5727..5946
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             complement(6410..7216)
                     /codon_start=1
                     /gene="rsvB"
                     /product="resolvase"
                     /function="Integrase"
                     /label=rsvB
                     /note="DNA binding site [nucleotide binding]; DNA 
                     breaking-rejoining enzymes, C-terminal catalytic domain. 
                     The DNA breaking-rejoining enzyme superfamily includes type
                     IB topoisomerases and tyrosine recombinases that share the 
                     same fold in their catalytic domain containing six 
                     conserved active site...; cl00213; Int/Topo IB signature 
                     motif; enteropathogenic; gene_5; identified by 
                     MetaGeneAnnotator; putative; resolvase [Escherichia coli]"
                     /protein_id="BAU61625.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMVH
                     DELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQ
                     LATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRRTGR
                     TEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVL
                     QSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(6410..7216)
                     /gene="rsvB"
                     /label=rsvB
     CDS             complement(7217..7522)
                     /codon_start=1
                     /product="plasmid maintenance protein CcdB"
                     /label=plasmid maintenance protein CcdB
                     /note="controlled cell death protein; post-segregation 
                     toxin; toxin addiction system; gene_6; identified by 
                     MetaGeneAnnotator; putative; plasmid maintenance protein 
                     CcdB; plasmid maintenance protein CcdB [Escherichia coli]; 
                     plasmid maintenance protein CcdB; Provisional"
                     /protein_id="BAU61626.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8097..8117
                     /label=pKS300IC_Fwd
                     /note="pKS300IC_Fwd"