LOCUS       Exported                7570 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKS145 DNA, complete sequence.
ACCESSION   AB013921
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Terada Y, Park H-S., Kuroki Y, Fujiyama A.
  TITLE     Cloning vector pKS145
  JOURNAL   Published Only in Database (1998)
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Terada Y, Park H-S., Kuroki Y, Fujiyama A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-1998) Contact:Asao Fujiyama National Institute of 
            Genetics, Human Genetics; Yata 1111, Mishima 411-8540, Japan
REFERENCE   3  (bases 1 to 7570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Dec 22, 2008 this sequence version replaced AB013921.1.
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Cloning vector pKS145"
                     /mol_type="other DNA"
                     /db_xref="taxon:76876"
     primer_bind     complement(73..89)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..106)
                     /label=pKS145_Fwd
                     /note="pKS145_Fwd"
     regulatory      complement(881..922)
                     /regulatory_class="terminator"
                     /note="contains the T4 gene 32 terminator and a portion of 
                     the polylinker from the omega interposon; omega terminator"
     CDS             940..1800
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="BAH03496.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            940..1800
                     /gene="bla"
                     /label=bla
     repeat_region   2596..3113
                     /function="plasmid partition centromere element"
                     /label=sopC
                     /rpt_family="SopB binding"
                     /note="sopC"
     misc_feature    2640..3113
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3186..4157)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein"
                     /function="plasmid partition"
                     /label=sopB
                     /protein_id="BAH03497.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(3186..4157)
                     /gene="sopB"
                     /label=sopB
     CDS             complement(4157..5332)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             complement(4157..5323)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein"
                     /function="plasmid partition"
                     /label=sopA
                     /protein_id="BAH03498.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGEA
                     ADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAEDV
                     FPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDLHIH
                     AEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTDPHL
                     MLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFFDML
                     RDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDEVGK
                     GQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(4157..5323)
                     /gene="sopA"
                     /label=sopA
     misc_feature    5658..5908
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     repeat_region   5661..5908
                     /function="copy number control"
                     /label=incC
                     /rpt_family="iteron, RepE binding"
                     /note="incC"
     CDS             complement(5911..6666)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiator protein"
                     /label=repE
                     /protein_id="BAH03499.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(5911..6666)
                     /gene="repE"
                     /label=repE
     rep_origin      6757..6976
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    7396..7417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7432..7462
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     7466..7486
                     /label=pKS145_Rev
                     /note="pKS145_Rev"
     protein_bind    7470..7486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7494..7510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7506..238
                     /codon_start=1
                     /gene="lacZa"
                     /product="beta-galactosidase alpha"
                     /function="beta-galactosidase alpha donor"
                     /label=lacZa
                     /note="contains a multiple cloning site incorporated into 
                     the 5 end of lacZ"
                     /protein_id="BAH03500.1"
                     /translation="MTMITPSARNTTHYRAAASMLESSADVVRREAFERVCIDHVDPAR
                     SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWKL"
     gene            7506..238
                     /gene="lacZa"
                     /label=lacZa
     promoter        7532..7550
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"