LOCUS       Exported                4320 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pKR147, complete sequence.
ACCESSION   KF528988
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Rossger K, Charpin-El Hamri G, Fussenegger M.
  TITLE     Reward-based hypertension control by a synthetic brain-dopamine 
            interface
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 110 (45), 18150-18155 (2013)
  PUBMED    24127594
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Roessger K, Fussenegger M, Charpin-El-Hamri G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, 
            BS 4058, Switzerland
REFERENCE   3  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Mammalian expression vector pKR147"
                     /mol_type="other DNA"
                     /db_xref="taxon:1419744"
     CDS             23..976
                     /codon_start=1
                     /product="ANP-IgG"
                     /label=ANP-IgG
                     /note="extendin-4-furin cleavage site-ANP-linker-mIgG-Fc"
                     /protein_id="AHA35650.1"
                     /translation="MKIILWLCVFGLFLATLFPISWQMPVESGLSSEDSASSESFAKRI
                     KRSLRRSSCFGGRIDRIGAQSGLGCNSFRYRRGSGGSGGSGGSGGSGGRSGCKPCICTV
                     PEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPRE
                     EQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIP
                     PPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLN
                     VQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK"
     polyA_signal    1016..1137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1541..2160
     rep_origin      complement(1556..2144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2315..3175)
                     /codon_start=1
                     /product="ampicillin resistance marker"
                     /label=ampicillin resistance marker
                     /protein_id="AHA35651.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3176..3280)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      3217..3245
                     /regulatory_class="promoter"
     rep_origin      3307..3762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      3324..3630
     polyA_signal    3893..3941
                     /note="synthetic polyadenylation signal"
     misc_feature    3955..4046
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     regulatory      4080..4319
                     /regulatory_class="promoter"
                     /note="CRE promoter"