LOCUS       Exported                4319 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKMAG-C, complete sequence.
ACCESSION   KX714577
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Bardaji L, Anorga M, Ruiz-Maso JA, Del Solar G, Murillo J.
  TITLE     Plasmid replicons from Pseudomonas are natural chimeras of 
            functional, exchangeable modules
  JOURNAL   Front Microbiol (2017) In press
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Anorga M, Bardaji L, Murillo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2016) Departamento de Produccion Agraria, 
            Universidad Publica de Navarra, Campus de Arrosadia, Pamplona 31006,
            Spain
REFERENCE   3  (bases 1 to 4319)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Cloning vector pKMAG-C"
                     /mol_type="other DNA"
                     /db_xref="taxon:1940342"
     misc_feature    join(1..46,2324..4319)
                     /note="derived from pK184; GI:392879"
     misc_feature    55..1795
                     /note="derived from pPsv48C (FR820587), containing the 
                     replicase gene, repA; GI:410686395"
     CDS             368..1681
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication initiator protein YP_006962014"
                     /protein_id="APZ73802.1"
                     /translation="MRPDHAISLAIATSPTANADPLASSTHLPPARFFEDGTALNRLLL
                     EAPYMARCSDDKTATRVRPREYALRYPYMQVNRPGMVSWLVFDLDHANALAWDDAGLPA
                     PNLMVRNRKSGHSQLFYAVPSVCTTENARAKPIQYMKAIYAAFAARLDADVDYHGGPVA
                     KTPGHPWWETTEFHSHVYELGELASAVELTVKPWATGPKLDQVSHSRHCILFELLRYFA
                     YSIVNRERELGSFESFMRSLDAYAYNHNSFLKQGFSENLPLSSIRATVKSVGRWTWDRY
                     TGDRRCHRGAMQLNSSLSLTERQSLAAKRTHELRHKATESKIRAACRQLQDQGKALVRS
                     AIAALAGVSASTVARYAHILSEVTKPATVSVLKVSADERPTVTPGDQAAPREAVQCLKK
                     QAMTDRDQGVSYAVHQISAVPQGPQAGESLKTEHEDGS"
     gene            368..1681
                     /gene="repA"
                     /label=repA
     misc_feature    1816..2262
                     /note="derived from pMe6041; GI:4378794"
     regulatory      1866..2132
                     /regulatory_class="terminator"
                     /note="T4 transcription terminator"
     misc_feature    2134..2244
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      2287..2296
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      2291..2300
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2618..3412
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin phosphotransferase"
                     /label=kan
                     /note="kanamycin resistance"
                     /protein_id="APZ73803.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2618..3412
                     /gene="kan"
                     /label=kan
     rep_origin      3558..4103
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."