LOCUS       Exported                6039 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transposon vector pkM225, complete sequence.
ACCESSION   HQ386859
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Mertens K, Berg JP, Brennan RE, Weber MM, Chen C, Samuel JE.
  TITLE     Evolutionary Loss of katE as Balance Mechanism for Antioxidant Gene 
            Expression in Coxiella burnetii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Mertens K, Berg JP, Brennan RE, Weber MM, Chen C, Samuel JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2010) Microbial and Molecular Pathogenesis, Texas 
            A&M Health Science Center, 407 Reynolds Medical Building, College 
            Station, TX 77843-1114, USA
REFERENCE   3  (bases 1 to 6039)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Transposon vector pkM225"
                     /lab_host="Coxiella burnetii"
                     /mol_type="other DNA"
                     /db_xref="taxon:928675"
     CDS             329..1375
                     /codon_start=1
                     /product="transposase TnA7"
                     /label=transposase TnA7
                     /protein_id="ADQ57456.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQQRVDDSERCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIRELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYRNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             1749..2558
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin resistance protein"
                     /label=kan
                     /protein_id="ADQ57457.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1749..2558
                     /gene="kan"
                     /label=kan
     primer_bind     complement(2630..2646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2796..2822
                     /note="IR; inverted repeat"
     rep_origin      2868..3581
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(2923..3511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             4313..5023
                     /codon_start=1
                     /product="red fluorescence protein mCherry"
                     /label=red fluorescence protein mCherry
                     /protein_id="ADQ57458.1"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             5310..5969
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /protein_id="ADQ57459.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            5310..5969
                     /gene="cat"
                     /label=cat
     repeat_region   6011..6037
                     /note="IR; inverted repeat"