LOCUS       Exported                6879 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mariner transposon vector pKKma, complete sequence.
ACCESSION   KJ933396
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Zhang C.
  TITLE     Characterization of a mariner transposon pKKma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Zhang C.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUN-2014) Biological Engineering, Tianjin University 
            of Science and Technology, Thirteen Avenue of Economic Development, 
            Tianjin 300457, China
REFERENCE   3  (bases 1 to 6879)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Mariner transposon vector pKKma"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /country="China"
                     /db_xref="taxon:1541884"
     rep_origin      91..479
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(415..1041)
                     /codon_start=1
                     /gene="traI"
                     /product="conjugal transfer relaxase"
                     /label=traI
                     /protein_id="AIN44219.1"
                     /translation="MRSIKKSDFAELVKYITDEQGKTERLGHVRVTNCEANTLPAVMAE
                     VMATQHGNTRSEADKTYHLLVSFRAGEKPDAETLRAIEDRICAGLGFAEHQRVSAVHHD
                     TDNLHIHIAINKIHPTRNTIHEPYRAYRALADLCATLERDYGLERDNHETRQRVSENRA
                     NDMERHAGVESLVGWIRPRCVRRRGSEDQQFNLLIVRTKLSCFTY"
     gene            complement(415..1041)
                     /gene="traI"
                     /label=traI
     misc_feature    complement(1097..1536)
                     /note="similar to TraJ; plasmid conjugative transfer 
                     protein"
     CDS             complement(1097..1468)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYRITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            1501..1610
                     /note="incP origin of transfer"
     misc_feature    1836..2231
                     /note="similar to TraK; oriT binding protein"
     primer_bind     complement(2229..2245)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2276..2380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2381..3241
                     /codon_start=1
                     /gene="bla"
                     /product="AmpR"
                     /label=bla
                     /note="ampicillin resistance protein"
                     /protein_id="AIN44220.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2381..3241
                     /gene="bla"
                     /label=bla
     CDS             3567..4613
                     /codon_start=1
                     /gene="tnpA"
                     /product="transposae"
                     /label=tnpA
                     /protein_id="AIN44221.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     gene            3567..4613
                     /gene="tnpA"
                     /label=tnpA
     promoter        complement(4675..4693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4703..4719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4851..4877
     CDS             complement(5053..5586)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamicin resistant protein"
                     /label=aacC1
                     /protein_id="AIN44222.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            complement(5053..5586)
                     /gene="aacC1"
                     /label=aacC1
     promoter        complement(5775..5803)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        6504..6532
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    6540..6556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     repeat_region   6612..6638