LOCUS       Exported                4040 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKK30, complete sequence.
ACCESSION   KX085043
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Krute CN, Krausz KL, Markiewicz MA, Joyner JA, Pokhrel S, Hall PR, 
            Bose JL.
  TITLE     Generation of a stable plasmid for in vitro and in vivo studies of 
            Staphylococcus
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
  PUBMED    27637878
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Krausz KL, Krute CN, Markiewicz MA, Bose JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2016) Microbiology, Molecular Genetics and 
            Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd, 
            Kansas City, KS 66160, USA
REFERENCE   3  (bases 1 to 4040)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Cloning vector pKK30"
                     /mol_type="other DNA"
                     /db_xref="taxon:1903329"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label=repF
                     /note="replication protein"
                     /protein_id="AOR52299.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label=repF
     rep_origin      1147..1570
                     /label=R6K origin
                     /note="R6K origin"
     rep_origin      1179..1567
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /regulatory_class="promoter"
                     /note="sarA P1 promoter"
     CDS             1847..2332
                     /codon_start=1
                     /gene="dfrA"
                     /product="DfrA"
                     /function="trimethoprim resistance"
                     /label=dfrA
                     /protein_id="AOR52300.1"
                     /translation="MTLSIIVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMAR
                     KTFNSIGKPLPNRRNVVLTNQASFRHEGVDVINSLDEIKELSGHVFIFGGQTLYEAMID
                     QVDDMYITVIDGKFQGDTFFPPYTFENWEVESSVEGQLDEKNTIPHTFLHLVRRKGK"
     gene            1847..2332
                     /gene="dfrA"
                     /label=dfrA
     regulatory      2340..2639
                     /regulatory_class="terminator"
                     /note="blaZ transcription terminator"
     regulatory      2766..2823
                     /regulatory_class="terminator"
                     /note="atl transcription terminator"
     rep_origin      3220..3397
                     /label=sso
                     /note="sso"
     rep_origin      3866..3886
                     /gene="dso"
     gene            3866..3886
                     /gene="dso"
                     /label=dso