LOCUS       Exported                4841 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKK22, complete sequence.
ACCESSION   KX085042
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Krute CN, Krausz KL, Markiewicz MA, Joyner JA, Pokhrel S, Hall PR, 
            Bose JL.
  TITLE     Generation of a stable plasmid for in vitro and in vivo studies of 
            Staphylococcus
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
  PUBMED    27637878
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Krausz KL, Krute CN, Markiewicz MA, Bose JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2016) Microbiology, Molecular Genetics and 
            Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd, 
            Kansas City, KS 66160, USA
REFERENCE   3  (bases 1 to 4841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Cloning vector pKK22"
                     /mol_type="other DNA"
                     /db_xref="taxon:1903328"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /function="replication"
                     /label=repF
                     /note="replication protein"
                     /protein_id="AOR52293.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label=repF
     rep_origin      1147..1570
                     /label=R6K ori
                     /note="R6K ori"
     rep_origin      1179..1567
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /regulatory_class="promoter"
                     /note="sarA P1 promoter"
     CDS             1847..2332
                     /codon_start=1
                     /gene="dfrA"
                     /product="DfrA"
                     /function="trimethoprim resistance"
                     /label=dfrA
                     /protein_id="AOR52294.1"
                     /translation="MTLSIIVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMAR
                     KTFNSIGKPLPNRRNVVLTNQASFRHEGVDVINSLDEIKELSGHVFIFGGQTLYEAMID
                     QVDDMYITVIDGKFQGDTFFPPYTFENWEVESSVEGQLDEKNTIPHTFLHLVRRKGK"
     gene            1847..2332
                     /gene="dfrA"
                     /label=dfrA
     regulatory      2340..2639
                     /regulatory_class="terminator"
                     /note="blaZ transcriptional terminator"
     regulatory      2766..2823
                     /regulatory_class="terminator"
                     /gene="atl"
     gene            2766..2823
                     /gene="atl"
                     /label=atl
     CDS             3064..3180
                     /codon_start=1
                     /gene="cds2"
                     /product="hypothetical protein"
                     /label=cds2
                     /protein_id="AOR52295.1"
                     /translation="MGSVKKLVTGFAEIFVCEITIYLFGSYLRFKSLYIFLS"
     gene            3064..3180
                     /gene="cds2"
                     /label=cds2
     CDS             3262..3483
                     /codon_start=1
                     /gene="cds3"
                     /product="hypothetical protein"
                     /label=cds3
                     /protein_id="AOR52296.1"
                     /translation="MIKFFKKGVFILNKNTVLDEGYVIATILNVFFLLGLIFISHLENL
                     YILIPYVILMGINAIYLVVKVMNFKKNN"
     gene            3262..3483
                     /gene="cds3"
                     /label=cds3
     rep_origin      3631..3808
                     /label=sso
                     /note="sso"
     CDS             complement(3809..4390)
                     /codon_start=1
                     /gene="cds4"
                     /product="hypothetical protein"
                     /label=cds4
                     /protein_id="AOR52297.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
     gene            complement(3809..4390)
                     /gene="cds4"
                     /label=cds4
     CDS             4465..4638
                     /codon_start=1
                     /gene="cds5"
                     /product="hypothetical protein"
                     /label=cds5
                     /protein_id="AOR52298.1"
                     /translation="MNKFFYFLIILLSVFLIFNFVNSILFDNPFDFLESFIYSLVFTLI
                     YLGLEGHKKKKE"
     gene            4465..4638
                     /gene="cds5"
                     /label=cds5
     rep_origin      4667..4687
                     /label=dso
                     /note="dso"