LOCUS       Exported                3444 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKITE301P DNA, complete sequence.
ACCESSION   LC317096
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Toda, H., Itoh, N.
  TITLE     Development of novel E. coli-Kocuria shuttle vector using cryptic 
            plasmid pKPAL3 from Kocuria palustris IPUFS-1 and utilization for 
            producing enantiopure (S)-styrene oxide
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Itoh N, Toda, H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural 
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu, 
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 3444)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Cloning vector pKITE301P"
                     /mol_type="other DNA"
                     /db_xref="taxon:2033404"
     CDS             331..633
                     /codon_start=1
                     /gene="lacZ-alpha"
                     /product="beta-glucosidase alpha fragment"
                     /label=lacZ-alpha
                     /protein_id="BBA31611.1"
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF"
     gene            331..633
                     /gene="lacZ-alpha"
                     /label=lacZ-alpha
     misc_feature    339..395
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(399..415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(634..1449)
                     /codon_start=1
                     /gene="kanR"
                     /product="kanamycin resistance protein"
                     /label=kanR
                     /protein_id="BBA31612.1"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(634..1449)
                     /gene="kanR"
                     /label=kanR
     rep_origin      1831..2419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2441..3444
                     /label=pKPAL3-derived plasmid replication origin
                     /note="pKPAL3-derived plasmid replication origin"