LOCUS       Exported                3066 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKfloxANTSAsc, complete sequence.
ACCESSION   JQ301472
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Yoon YG, Koob MD.
  TITLE     Nonreplicating Intracellular Bacterial Vector for Conjugative DNA 
            Transfer into Mitochondria
  JOURNAL   Pharm. Res. (2012) In press
  PUBMED    22350804
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Koob MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2011) Lab Medicine and Pathology, ITN and IHG, 
            University of Minnesota, 2101 6th St SE, WMBB, Minneapolis, MN 
            55455, USA
REFERENCE   3  (bases 1 to 3066)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Cloning vector pKfloxANTSAsc"
                     /mol_type="other DNA"
                     /db_xref="taxon:1151786"
     regulatory      44..315
                     /regulatory_class="terminator"
                     /note="phage lambda tl3 terminator"
     terminator      55..301
                     /label=-lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     misc_recomb     complement(319..707)
                     /note="phage lambda ti terminator and att recombination 
                     site"
     protein_bind    425..656
                     /gene="attP"
                     /label=-lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     rep_origin      complement(739..1675)
                     /direction=LEFT
                     /label=pBR322 replication ori
                     /note="pBR322 replication ori"
     rep_origin      complement(975..1563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(1703..1736)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     1703..1727
                     /label=loxP
                     /note="loxP"
     CDS             complement(1754..2548)
                     /codon_start=1
                     /product="aminoglycoside-3-O-phosphotransferase"
                     /label=aminoglycoside-3-O-phosphotransferase
                     /note="kanamycin resistance"
                     /protein_id="AFC75902.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(2689..2722)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     2689..2713
                     /label=loxP
                     /note="loxP"
     regulatory      2739..3002
                     /regulatory_class="terminator"
                     /note="rRNA terminator, E. coli"
     terminator      2790..2876
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2968..2995
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    3013..3
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"