LOCUS       Exported                5276 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKanCOs1, complete sequence.
ACCESSION   JF837313
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Oster CJ, Phillips GJ.
  TITLE     Vectors for ligation-independent construction of lacZ gene fusions 
            and cloning of PCR products using a nicking endonuclease
  JOURNAL   Plasmid 66 (3), 180-185 (2011)
  PUBMED    21854804
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Oster C, Phillips GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2011) Veterinary Microbiology, Iowa State 
            University, 1802 University Boulevard, Ames, IA 50010, USA
REFERENCE   3  (bases 1 to 5276)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Cloning vector pKanCOs1"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:1090869"
     primer_bind     complement(127..143)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      847..1069
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1117..2067
                     /codon_start=1
                     /gene="repA"
                     /product="Rep"
                     /label=repA
                     /protein_id="AEP39217.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            1117..2067
                     /gene="repA"
                     /label=repA
     CDS             complement(3314..4129)
                     /codon_start=1
                     /gene="aph"
                     /product="aminoglycoside 3-phosphotransferase"
                     /label=aph
                     /protein_id="AEP39218.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(3314..4129)
                     /gene="aph"
                     /label=aph
     rep_origin      complement(4575..5030)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5172..5188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5198..5216
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5242..5258
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"