LOCUS       Exported                3352 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKA1U5 DNA, complete sequence.
ACCESSION   AB191256
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Kato S, Ohtoko K, Ohtake H, Kimura T.
  TITLE     Vector-capping: a simple method for preparing a high-quality 
            full-length cDNA library
  JOURNAL   DNA Res. 12 (1), 53-62 (2005)
  PUBMED    16106752
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Kato S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2004) Contact:Seishi Kato National Rehabilitation 
            Center for Persons with Disabilities, Research Institute, Department
            of Rehabilitation Engineering; 4-1 Namiki, Tokorozawa, Saitama 
            359-8555, Japan
REFERENCE   3  (bases 1 to 3352)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Cloning vector pKA1U5"
                     /mol_type="other DNA"
                     /label=full-length cDNA cloning vector pKA1U5
                     /note="full-length cDNA cloning vector pKA1U5"
                     /db_xref="taxon:295032"
     promoter        18..347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      31..241
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      280
                     /label=SV40 origin
                     /note="SV40 origin"
     intron          386..482
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     526..542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        550..568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      550..566
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     misc_feature    588..593
                     /label=EcoRV site for cDNA cloning
                     /note="EcoRV site for cDNA cloning"
     misc_feature    596..601
                     /label=KpnI site for dT tailing
                     /note="KpnI site for dT tailing"
     promoter        complement(617..635)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    637..771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1069..1657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1828..2688)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="BAD52432.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2689..2793)
                     /gene="bla"
                     /label=AmpR promoter
     misc_structure  2820..3275
                     /label=f1 phage IG region
                     /note="f1 phage IG region"
     rep_origin      complement(2820..3275)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3293..3309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"