LOCUS       Exported                3793 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pK19mob DNA, complete sequence.
ACCESSION   LC257598
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Okamoto S, Niki H.
  TITLE     NBRP cloning vector collection sequence project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima, 
            Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 3793)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Cloning vector pK19mob"
                     /mol_type="other DNA"
                     /db_xref="taxon:1979257"
     CDS             313..1107
                     /codon_start=1
                     /gene="aph(3)-II"
                     /product="confers resistance to neomycin, kanamycin, and 
                     G418"
                     /label=aph(3)-II
                     /protein_id="BAX25489.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            313..1107
                     /gene="aph(3)-II"
                     /label=aph(3)-II
     oriT            1445..1554
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     promoter        complement(1789..1879)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        complement(2073..2163)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2523..3111
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3399..3420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      3435..3465
                     /regulatory_class="promoter"
                     /gene="lac promoter"
                     /note="promoter for the E. coli lac operon"
     gene            3435..3465
                     /gene="lac promoter"
                     /label=lac promoter
     promoter        3435..3465
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3473..3489
                     /gene="lac operator"
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     gene            3473..3489
                     /gene="lac operator"
                     /label=lac operator
     primer_bind     3497..3513
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3509..3793
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZalpha fragment of beta-galactosidase"
                     /label=lacZ fragment
                     /protein_id="BAX25490.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDKLASRCRKHSGRKGC"
     gene            3509..3793
                     /gene="lacZ fragment"
                     /label=lacZ fragment
     misc_feature    3526..3582
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3583..3599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"