LOCUS       Exported                3053 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pK18PolyF2, complete sequence.
ACCESSION   AY222811
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J.
  TITLE     Efficient electrotransformation of corynebacterium diphtheriae with 
            a mini-replicon derived from the Corynebacterium glutamicum plasmid 
            pGA1
  JOURNAL   Curr. Microbiol. 45 (5), 362-367 (2002)
  PUBMED    12232668
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 3053)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Cloning vector pK18PolyF2"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:224892"
     primer_bind     complement(79..95)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             602..1396
                     /codon_start=1
                     /gene="aph(3)-IIa"
                     /product="Aph(3)-IIa"
                     /label=aph(3)-IIa
                     /note="aminoglyoside phosphotransferase"
                     /protein_id="AAO65976.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            602..1396
                     /gene="aph(3)-IIa"
                     /label=aph(3)-IIa
     oriT            1734..1843
                     /note="incP origin of transfer"
     misc_feature    1781..1879
                     /label=RP4-mob region
                     /note="RP4-mob region"
     rep_origin      2055..2643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2642..2644
                     /label=oriV
                     /note="oriV"
     protein_bind    2931..2952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2967..2997
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3005..3021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3029..3045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"