LOCUS       Exported                4589 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pK-Sul1, complete sequence.
ACCESSION   HQ593859
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection 
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
  PUBMED    21366919
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research 
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Cloning vector pK-Sul1"
                     /mol_type="other DNA"
                     /db_xref="taxon:980182"
     regulatory      complement(21..180)
                     /regulatory_class="terminator"
                     /note="CaMV35S polyA terminator"
     CDS             complement(236..1177)
                     /codon_start=1
                     /gene="Sul1"
                     /product="Sul1 dihydropteroate synthase precursor"
                     /label=Sul1
                     /note="sulfonamide resistance protein; confers resistance 
                     to sulfadiazine in plants"
                     /protein_id="ADW81558.1"
                     /translation="MGPFGGLKSMTGFPVKKVNTDITSITSNGGRVKCMVTVFGILNLT
                     EDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVGPAASHPDARPVSPADEIRRIAPLLD
                     ALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQGFPDPALYPDIAEADCRLVVMHSAQ
                     RDGIATRTGHLRPEDALDEIVRFFEARVSALRRSGVAADRLILDPGMGFFLSPAPETSL
                     HVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLPVKDLGPASLAAELHAIGNGADYVRT
                     HAPGDLRSAITISETLAKFRSRDARDRGLDHA"
     gene            complement(236..1177)
                     /gene="Sul1"
                     /label=Sul1
                     /note="SUL1"
     transit_peptide complement(1076..1177)
                     /gene="Sul1"
                     /note="pea rbcS ribulose-bisphosphate carboxylase small 
                     subunit transit peptide for plastid targeting"
     regulatory      complement(1184..1930)
                     /regulatory_class="promoter"
                     /note="double enhanced CaMV35S promoter"
     promoter        complement(1184..1528)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1951..4589
                     /label=pUC19 backbone
                     /note="pUC19 backbone"
     primer_bind     complement(1963..1979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1987..2003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2011..2041)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2056..2077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2365..2953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3124..3984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3985..4089)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4570..4586
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"