LOCUS       Exported                8709 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pJZ222, complete sequence.
ACCESSION   KP244678
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Pest control. Full crop protection from an insect pest by expression
            of long double-stranded RNAs in plastids
  JOURNAL   Science 347 (6225), 991-994 (2015)
  PUBMED    25722411
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular 
            Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, 
            Germany
REFERENCE   3  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Chloroplast transformation vector pJZ222"
                     /mol_type="other DNA"
                     /db_xref="taxon:1622245"
     tRNA            31..104
                     /gene="trn-fM"
                     /product="tRNA-Met"
                     /label=trn-fM
     gene            31..104
                     /gene="trn-fM"
                     /label=trn-fM
     CDS             complement(254..556)
                     /codon_start=1
                     /gene="rps14"
                     /product="ribosomal protein S14"
                     /label=rps14
                     /protein_id="AJR21482.1"
                     /translation="MARKSLIQREKKRQKLEQKYHSIRRSSKKEISKVPSLSDKWEIYG
                     KLQSPPRNSAPTRLHRRCFLTGRPRANYRDFGLSGHILREMVHACLLPGATRSSW"
     gene            complement(254..556)
                     /gene="rps14"
                     /label=rps14
     misc_feature    complement(679..1879)
                     /label=3 part of psaB
                     /note="3 part of psaB"
     promoter        complement(1891..1909)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1919..1935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2077..2532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2558..2662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2663..3523
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="AJR21483.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2663..3523
                     /gene="bla"
                     /label=bla
     rep_origin      3694..4282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4570..4591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4606..4636
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4644..4660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4668..4684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4705..4723
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4737..4867
                     /label=3 part of psbZ
                     /note="3 part of psbZ"
     tRNA            5143..5213
                     /gene="trnG"
                     /product="tRNA-Gly"
                     /label=trnG
     gene            5143..5213
                     /gene="trnG"
                     /label=trnG
     primer_bind     5418..5434
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      5443..5559
                     /regulatory_class="promoter"
                     /gene="Prrn"
     gene            5443..5559
                     /gene="Prrn"
                     /label=Prrn
     misc_feature    5572..5868
                     /note="actin; similar to Leptinotarsa decemlineata 
                     beta-actin"
     misc_feature    5869..6088
                     /note="shrub; similar to Leptinotarsa decemlineata shrub 
                     gene"
     intron          6096..6296
                     /gene="St-GA20 ox"
                     /number=1
                     /note="first intron from potato GA20 oxidase gene"
     gene            6096..6296
                     /gene="St-GA20 ox"
                     /label=St-GA20 ox
                     /note="GA20 oxidase"
     misc_feature    complement(6310..6529)
                     /label=similar to Leptinotarsa decemlineata shrub gene
                     /note="similar to Leptinotarsa decemlineata shrub gene"
     misc_feature    complement(6530..6826)
                     /label=similar to Leptinotarsa decemlineata actin gene
                     /note="similar to Leptinotarsa decemlineata actin gene"
     regulatory      6833..7051
                     /regulatory_class="terminator"
                     /note="E.coli rrnB terminator"
     terminator      6835..6921
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7013..7040
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(7071..7087)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      7112..7387
                     /regulatory_class="promoter"
                     /gene="PpsbA"
                     /note="similar to Chlamydomonas reinhardtii psbA gene"
     gene            7112..7387
                     /gene="PpsbA"
                     /label=PpsbA
     CDS             7394..8185
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside 3-adenylyltransferase"
                     /function="spectionmycin resistance"
                     /label=aadA
                     /protein_id="AJR21484.1"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            7394..8185
                     /gene="aadA"
                     /label=aadA
     3UTR           8200..8635
                     /gene="rbcL"
                     /label=similar to Chlamydomonas reinhardtii rbcL gene
                     /note="similar to Chlamydomonas reinhardtii rbcL gene"
     gene            8200..8635
                     /gene="rbcL"
                     /label=rbcL
     promoter        complement(8656..8674)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"