LOCUS       Exported                3839 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJW3, complete sequence.
ACCESSION   JX456191
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Javid B, Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2012) Centre for Infectious Diseases Research, 
            Tsinghua University School of Medicine, Lab 4303, Biotechnology 
            Building, Tsinghua University, Haidian District, Beijing, Beijing 
            100084, China
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Cloning vector pJW3"
                     /mol_type="other DNA"
                     /db_xref="taxon:1242764"
     rep_origin      7..1902
                     /label=oriM
                     /note="oriM"
     regulatory      1909..2053
                     /regulatory_class="promoter"
                     /note="TB HSP70 promoter"
     CDS             2054..2497
                     /codon_start=1
                     /product="Ble"
                     /label=Ble
                     /note="zeocin resistance gene-FLAG tagged"
                     /protein_id="AFV14773.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQDDYKDDDDKAANDENYSENYADAS"
     CDS             2054..2425
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             2426..2449
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      2504..2884
                     /label=oriR6K
                     /note="oriR6K"
     rep_origin      2507..2863
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     complement(2897..2913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3026..3835
                     /codon_start=1
                     /product="aph"
                     /label=aph
                     /note="kanamycin kinase"
                     /protein_id="AFV14772.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"