LOCUS       Exported                9885 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJV53-GFP, complete sequence.
ACCESSION   KU306402
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Mao X-J., Yan M-Y., Zhu H, Guo X-P., Sun Y-C.
  TITLE     Efficient and simple generation of multiple unmarked gene deletions 
            in Mycobacterium smegmatis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Mao X-J., Yan M-Y., Zhu H, Guo X-P., Sun Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) Microbiology, Institute of Pathogen 
            Biology,Chinese Academy of Medical Sciences & Peking Union Medical 
            College (CAMS&PUMC), building 9, Beijing University Of Technology, 
            Yizhuang Development Zone, Daxing District, Beijing 100000, China
REFERENCE   3  (bases 1 to 9885)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Cloning vector pJV53-GFP"
                     /lab_host="mycobacterium and E.coli"
                     /mol_type="other DNA"
                     /isolation_source="E.coli"
                     /note="gp60 and gp61 inducing expression and gfp expression
                     vector"
                     /db_xref="taxon:1812984"
     terminator      3334..3380
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             3541..4356
                     /codon_start=1
                     /gene="aph"
                     /product="aph"
                     /label=aph
                     /note="kanamycin resistance"
                     /protein_id="AMQ45835.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            3541..4356
                     /gene="aph"
                     /label=aph
     CDS             4865..5581
                     /codon_start=1
                     /gene="gfp"
                     /product="gfp"
                     /label=gfp
                     /protein_id="AMQ45836.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            4865..5581
                     /gene="gfp"
                     /label=gfp
     rep_origin      5653..6241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"