LOCUS       Exported                6771 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJPVCS, complete sequence.
ACCESSION   MG191280
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Schmelling N, Axmann IM, Dienst D.
  TITLE     The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis
            sp. PCC 6803 as prokaryotic platforms for biosynthesis of cyclic 
            plant triterpenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Schmelling N, Axmann IM, Dienst D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Department of Chemistry - Microbial 
            Chemistry, Angstrom and Science for Life Laboratory, Lagerhyddsvagen
            1, Uppsala 75120 Uppsala, Sweden
REFERENCE   3  (bases 1 to 6771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Cloning vector pJPVCS"
                     /mol_type="other DNA"
                     /db_xref="taxon:2057152"
     regulatory      305..323
                     /regulatory_class="promoter"
                     /note="bacteriophage T7 RNA polymerase"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    384..405
                     /note="BioBrick prefix for parts that do not start with 
                     ATG"
     CDS             complement(407..1519)
                     /codon_start=1
                     /gene="merR"
                     /product="MerR family transcriptional regulator"
                     /label=merR
                     /note="cobalt-dependent transcriptional regulator from 
                     Synechocystis sp. PCC 6803; coaR"
                     /protein_id="ATY93172.1"
                     /translation="MKTNHLTIKELTDAVGGGVTPRMVRHYHTLGLLPPVQRSEGNYRL
                     YTQQDVQRLQRVIALKQQGFQLSHIRQLLDSHSEESLDPTLMVQLQQQYQAVIQQITRL
                     RQTASALEGLLGRDQSCQITQAEALAQLKQLDVDVQEGLGKLDQLWTNLDAETTTHPEA
                     FQESLKHLLPDLSAYSEITIHLLHQLVLACGDVSLVNAVRLSQGAIASARDALKAGCPV
                     VTDVPVVAAALDQTRLAHLGCTVKTLIDDPHITGLREAEQAFWHHDHWQQRLQQIPQGC
                     VLAIGYAPSVLLTACKLIEQQHIQPALVIGMPIGFSHAPGAKRRLMTSPIPHITIQGSL
                     GGGLLAAVTLNALVETLIAKPDCHCYLTCL"
     gene            complement(407..1519)
                     /gene="merR"
                     /label=merR
     regulatory      1520..1600
                     /regulatory_class="promoter"
                     /note="promoter including 5 UTR of coaT from Synechocystis
                     sp. PCC 6803 (Moscow wildtype)"
     CDS             1601..2320
                     /codon_start=1
                     /product="venus YFP"
                     /label=venus YFP
                     /note="mammalian codon-optimized; Venus"
                     /protein_id="ATY93173.1"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2329..2400
                     /regulatory_class="terminator"
                     /note="transcription terminator T1 from E. coli rrnB"
     terminator      2329..2400
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      2416..2443
                     /regulatory_class="terminator"
                     /note="phage T7 early transcription terminator; T7Te 
                     terminator"
     terminator      2416..2443
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     gene            complement(2462..3169)
                     /gene="nrsR"
                     /label=nrsR
     CDS             complement(2465..3169)
                     /codon_start=1
                     /gene="nrsR"
                     /product="OmpR subfamily"
                     /label=nrsR
                     /note="redox-responsive and/or Ni(II)-responsive regulator,
                     two-component response regulator OmpR; nrsR"
                     /protein_id="ATY93174.1"
                     /translation="MRILLVEDETDLGMAIKKVLVSEKYVVDWVTDGSQAWDYLENQWT
                     EYTLAIVDWLLPGLSGLELCQKLRTQGNSLPVLMLTALGEPENRVEGLDAGADDYLTKP
                     FVMAELLARLRALQRRSPQFQPQILTLGNFSLDPSNNLLSVTISEPLNLERQEIALTVR
                     EFQIFQYLMQNPERIISGSKIRQQLWDLDEEPMSNVVAAQMRLIRRKLAQQNCPCPIKT
                     VPGQGYRFTLSP"
     CDS             3288..4007
                     /codon_start=1
                     /product="cerulean fluorescent reporter"
                     /label=cerulean fluorescent reporter
                     /protein_id="ATY93175.1"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3753..4004
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     regulatory      4016..4087
                     /regulatory_class="terminator"
                     /note="transcription terminator T1 from E. coli rrnB"
     terminator      4016..4087
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      4103..4130
                     /regulatory_class="terminator"
                     /note="phage T7 early transcription terminator; T7Te 
                     terminator"
     terminator      4103..4130
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4137..4157
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
     regulatory      complement(4603..4633)
                     /regulatory_class="promoter"
                     /note="E. coli lac promoter with an up mutation; lac UV5 
                     promoter"
     promoter        complement(4603..4633)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    4648..4669
                     /label=catabolite activator protein binding site
                     /bound_moiety="catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP; CAP binding site"
     rep_origin      complement(4960..5548)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication; ColE1_ori"
     gene            complement(5719..6684)
                     /gene="bla"
                     /label=bla
     CDS             complement(5719..6579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics; AmpR"
                     /protein_id="ATY93176.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(6580..6684)
                     /regulatory_class="promoter"
                     /gene="bla"
     promoter        complement(6580..6684)
                     /gene="bla"
                     /label=AmpR promoter