LOCUS       Exported                3126 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pJP5603, complete sequence.
ACCESSION   JQ769377
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Riedel T, Rohlfs M, Buchholz I, Wagner-Dobler I, Reck M.
  TITLE     Complete sequence of the suicide vector pJP5603
  JOURNAL   Plasmid 69 (1), 104-107 (2013)
  PUBMED    22902299
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Riedel T, Reck M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Microbial Communication, Helmholtz-Centre 
            for Infection Research, Inhoffenstrasse 7, Braunschweig, Lower 
            Saxony 38124, Germany
REFERENCE   3  (bases 1 to 3126)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Suicide vector pJP5603"
                     /mol_type="other DNA"
                     /db_xref="taxon:1195379"
     CDS             204..998
                     /codon_start=1
                     /product="aminoglycoside-3-O-phosphotransferase"
                     /label=aminoglycoside-3-O-phosphotransferase
                     /protein_id="AFK93530.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    1273..1294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1309..1339
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1347..1363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1371..1387
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1383..1652
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWGSIPSRVGSAAA"
     misc_feature    1400..1456
                     /label=M13mp19 polylinker
                     /note="M13mp19 polylinker"
     primer_bind     complement(1457..1473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1779..2552
                     /label=RP4 mob site
                     /note="RP4 mob site"
     CDS             complement(1905..2276)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2309..2418
                     /note="incP origin of transfer"
     rep_origin      2578..2969
                     /label=R6K origin of replication
                     /note="R6K origin of replication"
     rep_origin      2578..2966
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             2966..97
                     /codon_start=1
                     /product="truncated transposase"
                     /label=truncated transposase
                     /protein_id="AFK93529.1"
                     /translation="MGISFTLPQALRAQGLLKEAEHVESQSAETVLTPDECQLLGYLDK
                     GKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW"