LOCUS       Exported                4376 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJLS298, complete sequence.
ACCESSION   MG752897
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Stoudenmire JL, Essock-Burns T, Weathers EN, Solaimanpour S, Mrazek 
            J, Stabb EV.
  TITLE     An iterative synthetic approach to engineer a high-performing 
            PhoB-specific reporter
  JOURNAL   Appl. Environ. Microbiol. (2018) In press
  PUBMED    29752265
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Stoudenmire JL, Stabb EV.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2018) Microbiology, University of Georgia, 120 
            Cedar Street, Athens, GA 30605, USA
REFERENCE   3  (bases 1 to 4376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Cloning vector pJLS298"
                     /mol_type="other DNA"
                     /db_xref="taxon:2212378"
     CDS             62..535
                     /codon_start=1
                     /product="trimethoprim resistance protein"
                     /label=trimethoprim resistance protein
                     /note="Drf1"
                     /protein_id="AWO14318.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVGR
                     KTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEIYK
                     SLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     CDS             complement(578..1288)
                     /codon_start=1
                     /product="red florescent protein MCherry"
                     /label=red florescent protein MCherry
                     /protein_id="AWO14319.1"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1390..1406
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1587..2339
                     /codon_start=1
                     /product="destabilized GFP"
                     /label=destabilized GFP
                     /note="GFP-ssrA"
                     /protein_id="AWO14320.1"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYNVANDENYAASV"
     promoter        complement(2380..2398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2414..2802
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            4075..4184
                     /note="incP origin of transfer"