LOCUS       Exported                8525 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJLO19, complete sequence.
ACCESSION   KP636798
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Lo J, Zheng T, Hon S, Olson DG, Lynd LR.
  TITLE     The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is 
            necessary for ethanol production in Clostridium thermocellum and 
            Thermoanaerobacterium saccharolyticum
  JOURNAL   J. Bacteriol. (2015) In press
  PUBMED    25666131
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Lo J, Zheng T, Hon S, Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2015) Thayer School of Engineering, Dartmouth 
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Cloning vector pJLO19"
                     /mol_type="other DNA"
                     /label=derived from Ruminiclostridium thermocellum
                     /note="derived from Ruminiclostridium thermocellum"
                     /db_xref="taxon:1615845"
     CDS             complement(558..1418)
                     /codon_start=1
                     /gene="bla"
                     /product="bla"
                     /EC_number="3.5.2.6"
                     /label=bla
                     /note="beta-lactamase"
                     /protein_id="AJO16024.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(558..1418)
                     /gene="bla"
                     /label=bla
     promoter        complement(1419..1523)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3192..3842
                     /codon_start=1
                     /gene="cat"
                     /product="cat"
                     /EC_number="2.3.1.28"
                     /label=cat
                     /note="chloramphenicol acetyltransferase"
                     /protein_id="AJO16026.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            3192..3842
                     /gene="cat"
                     /label=cat
     CDS             3860..4405
                     /codon_start=1
                     /gene="hpt"
                     /product="hpt"
                     /EC_number="2.4.2.8"
                     /label=hpt
                     /note="hypoxanthine phosphoribosyltransferase"
                     /protein_id="AJO16025.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            3860..4405
                     /gene="hpt"
                     /label=hpt
     rep_origin      complement(5571..6159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6246..6824)
                     /codon_start=1
                     /gene="tdk"
                     /product="tdk"
                     /EC_number="2.7.1.21"
                     /label=tdk
                     /note="thymidine kinase"
                     /protein_id="AJO16027.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(6246..6824)
                     /gene="tdk"
                     /label=tdk
     CDS             complement(7521..8525)
                     /codon_start=1
                     /gene="repB"
                     /product="repB"
                     /EC_number="3.6.4.12"
                     /label=repB
                     /note="replication protein"
                     /protein_id="AJO16028.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            complement(7521..8525)
                     /gene="repB"
                     /label=repB