LOCUS       Exported                4457 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJL-SKX, complete sequence.
ACCESSION   EF526067
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ludu JS, Nix EB, Duplantis BN, de Bruin OM, Gallagher LA, Hawley LM,
            Nano FE.
  TITLE     Genetic elements for selection, deletion mutagenesis and 
            complementation in Francisella spp
  JOURNAL   FEMS Microbiol. Lett. 278 (1), 86-93 (2008)
  PUBMED    18021237
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Nano FE, Ludu JS, Nix EB.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2007) Biochemistry and Microbiology, University of
            Victoria, 3800 Finnerty Rd., Victoria, British Columbia V8W 3P6, 
            Canada
REFERENCE   3  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Cloning vector pJL-SKX"
                     /lab_host="Francisella tularensis subsp. novicida"
                     /mol_type="other DNA"
                     /label=integrating complementation vector
                     /note="integrating complementation vector"
                     /db_xref="taxon:436668"
     promoter        29..47
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     84..100
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2037..2852
                     /codon_start=1
                     /gene="aph"
                     /product="Aph"
                     /label=aph
                     /note="kanamycin resistance marker; aminoglycoside 
                     3-phosphotransferase"
                     /protein_id="ABV03169.1"
                     /translation="MSHIQRETSWSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            2037..2852
                     /gene="aph"
                     /label=aph
     misc_feature    2853..2858
                     /note="unique XhoI site for introduction of genetic 
                     material"
     promoter        complement(4430..4448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"