LOCUS       Exported                4791 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJK95, complete sequence.
ACCESSION   AY738638
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Blodgett JA, Zhang JK, Metcalf WW.
  TITLE     Molecular cloning, sequence analysis, and heterologous expression of
            the phosphinothricin tripeptide biosynthetic gene cluster from 
            Streptomyces viridochromogenes DSM 40736
  JOURNAL   Antimicrob. Agents Chemother. 49 (1), 230-240 (2005)
  PUBMED    15616300
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Blodgett JAV., Zhang JK, Metcalf WW.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2004) Microbiology, University of Illinois, 601 S.
            Goodwin, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Cloning vector pJK95"
                     /mol_type="other DNA"
                     /db_xref="taxon:293597"
     mobile_element  20..3310
                     /mobile_element_type="transposon:mini-Mu"
     repeat_region   20..40
                     /label=derived from bacteriophage Mu
                     /note="derived from bacteriophage Mu"
     CDS             complement(101..910)
                     /codon_start=1
                     /gene="tsr"
                     /product="thiostrepton resistance protein"
                     /label=tsr
                     /note="RNA methylase"
                     /protein_id="AAU44620.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     gene            complement(101..910)
                     /gene="tsr"
                     /label=tsr
     promoter        1746..2075
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1926..2061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2436..3230
                     /codon_start=1
                     /gene="aph"
                     /product="kanamycin resistance protein"
                     /label=aph
                     /note="aminoglycoside phosphotransferase"
                     /protein_id="AAU44621.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2436..3230
                     /gene="aph"
                     /label=aph
     mobile_element  3290..3310
                     /mobile_element_type="transposon:inverted"
                     /note="derived from bacteriophage Mu"
     rep_origin      3351..3739
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3801..4661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAU44622.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3801..4661)
                     /gene="bla"
                     /label=bla
     promoter        complement(4662..4766)
                     /gene="bla"
                     /label=AmpR promoter