LOCUS       Exported                3854 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Allelic exchange vector pJK100, complete sequence.
ACCESSION   DQ157769
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Denef VJ, Klappenbach JA, Patrauchan MA, Florizone C, Rodrigues JL, 
            Tsoi TV, Verstraete W, Eltis LD, Tiedje JM.
  TITLE     Genetic and Genomic Insights into the Role of Benzoate-Catabolic 
            Pathway Redundancy in Burkholderia xenovorans LB400
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 585-595 (2006)
  PUBMED    16391095
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Denef VJ, Klappenbach JA, Patrauchan MA, Florizone C, Rodrigues JM, 
            Tsoi TV, Verstraete W, Eltis LD, Tiedje JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2005) Center for Microbial Ecology, Michigan State
            University, 540 Plant & Soil Sciences Building, East Lansing, MI 
            48824, USA
REFERENCE   3  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     pJK100 was derived from the 1674 bp BsgI-AatII fragment of pCM184 
            (Marx & Lidstrom, 2002) and the 2180 bp EcoRI-KpnI fragment of 
            pKNOCK-Tc (Alexeyev, 1999) that were blunt-eded and ligated 
            together. The vector was sequenced in both directions.
            Name: allelic exchange vector pJK100.
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Allelic exchange vector pJK100"
                     /mol_type="other DNA"
                     /note="pJK100 was derived from the 1674 bp BsgI-AatII 
                     fragment of pCM184 (Marx & Lidstrom, 2002) and the 2180 bp 
                     EcoRI-KpnI fragment of pKNOCK-Tc (Alexeyev, 1999) that were
                     blunt-ended and ligated together"
                     /db_xref="taxon:341830"
     protein_bind    547..580
                     /label=loxP binding site
                     /bound_moiety="loxP"
                     /note="cre recombinase recognition site; loxP recombination
                     site"
     rep_origin      881..1269
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     rep_origin      989..1048
                     /label=R6K origin of replication
                     /note="R6K origin of replication"
     oriT            1440..1549
                     /note="incP origin of transfer"
     oriT            1443..1502
                     /label=RP4 origin of transfer
                     /note="RP4 origin of transfer"
     CDS             1749..2939
                     /codon_start=1
                     /gene="tetR"
                     /product="TetR"
                     /label=tetR
                     /note="tetracycline resistance"
                     /protein_id="AAZ81616.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            1749..2939
                     /gene="tetR"
                     /label=tetR
                     /note="tetracycline resistance cassette"
     protein_bind    3108..3141
                     /label=loxP binding site
                     /bound_moiety="loxP"
                     /note="cre recombinase recogition site; loxP recombination 
                     site"
     CDS             3275..236
                     /codon_start=1
                     /gene="kanR"
                     /product="KanR"
                     /label=kanR
                     /note="kanamycin resistance"
                     /protein_id="AAZ81615.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            3275..236
                     /gene="kanR"
                     /label=kanR
                     /note="kanamycin resistance cassette"