LOCUS       Exported                4107 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast shuttle vector pJHU2, complete sequence.
ACCESSION   KF192692
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Annaluru N.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2013) Environmental Health Sciences, Johns Hopkins
            School of Public Health, 615 North Wolfe St. E6402, Baltimore, MD 
            21205, USA
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Yeast shuttle vector pJHU2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1400858"
     rep_origin      125..628
                     /note="CEN/ARS; Saccharomyces cerevisiae CEN6 centromere 
                     fused to an autonomously replicating sequence"
     misc_feature    125..628
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        891..1106
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             1107..1910
                     /codon_start=1
                     /gene="URA3"
                     /product="URA3"
                     /function="catalyzes the sixth enzymatic step in the de 
                     novo biosynthesis of pyrimidines, converting OMP into 
                     uridine monophosphate (UMP)"
                     /label=URA3
                     /note="orotidine-5-phosphate (OMP) decarboxylase; converts
                     5-FOA into 5-fluorouracil (a toxic compound)"
                     /protein_id="AGV98847.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            1107..1910
                     /gene="URA3"
                     /label=URA3
                     /note="YEL021W"
     protein_bind    2078..2094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2102..2118
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2114..2353
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPEFELGTRGSSRVDLQACKLGLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR"
     misc_feature    2132..2188
                     /label=pUC18/19 multiple cloning site
                     /note="pUC18/19 multiple cloning site"
     promoter        2398..2489
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3354..3393
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      3425..4013
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4054..4075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."