LOCUS       Exported                5477 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJG102, complete sequence.
ACCESSION   KX499654
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of 
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics, 
            University of Maryland, 3112 Bioscience Research Building, College 
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 5477)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Cloning vector pJG102"
                     /mol_type="other DNA"
                     /country="USA"
                     /note="for ectopic integration into the Bacillus 
                     amyloliquefaciens chromosome."
                     /db_xref="taxon:1930841"
     primer_bind     379..395
                     /note="M13 fwd; common sequencing primer, one of multiple 
                     similar variants"
     misc_feature    396..1005
                     /note="loaP region homology; loaP region homology 
                     containing dfnA and its leader region"
     regulatory      complement(1006..1164)
                     /regulatory_class="promoter"
                     /note="Pconst"
     CDS             1615..2349
                     /codon_start=1
                     /gene="erm"
                     /product="hypothetical protein"
                     /label=erm
                     /note="ErmR"
                     /protein_id="APU90125.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
     gene            1615..2349
                     /gene="erm"
                     /label=erm
     misc_feature    2443..3212
                     /note="LoaP Region Homology; homology region downstream of 
                     loaP"
     gene            2443..2473
                     /pseudo
                     /gene="loaP"
                     /label=loaP
                     /note="3; fragment of loaP left after marker replacement"
     primer_bind     complement(3256..3272)
                     /note="M13 rev; common sequencing primer, one of multiple 
                     similar variants"
     protein_bind    3280..3296
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator; The lac repressor binds to the lac 
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(3304..3334)
                     /regulatory_class="promoter"
                     /note="lac promoter; promoter for the E. coli lac operon"
     promoter        complement(3304..3334)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3349..3370
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site; CAP binding activates 
                     transcription in the presence of cAMP."
     rep_origin      complement(3658..4246)
                     /direction=LEFT
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     of replication"
     CDS             complement(4417..5277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampR; gene=bla - note=ampicillin resistance - 
                     codon_start=1 - transl_table=11 - product=beta-lactamase - 
                     protein_id=AAB40303.1 - db_xref=GI:1185570 - 
                     translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY - 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAG"
                     /protein_id="APU90126.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4417..5277)
                     /gene="bla"
                     /label=bla
     regulatory      complement(5278..5382)
                     /regulatory_class="promoter"
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        complement(5278..5382)
                     /gene="bla"
                     /label=AmpR promoter