LOCUS       Exported               10818 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJG095, complete sequence.
ACCESSION   KX499656
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of 
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics, 
            University of Maryland, 3112 Bioscience Research Building, College 
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 10818)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Cloning vector pJG095"
                     /mol_type="other DNA"
                     /country="USA"
                     /note="for ectopic integration into the Bacillus 
                     amyloliquefaciens chromosome."
                     /db_xref="taxon:1930836"
     CDS             complement(1..783)
                     /codon_start=1
                     /gene="spc"
                     /product="spectinomycin adenyltransferase"
                     /label=spc
                     /note="spectinomycin adenyltransferase; gene=spc - 
                     note=spectinomycin resistance - codon_start=1 - 
                     transl_table=11 - product=spectinomycin adenyltransferase -
                     protein_id=AAB40302.1 - db_xref=GI:1185569 - 
                     translation=MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI - 
                     NSDVDVLVVVNHSLPQLTRKK"
                     /protein_id="APU90129.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1..783)
                     /gene="spc"
                     /label=spc
     misc_feature    1892..2892
                     /pseudo
                     /note="amyE; amyE back; amyE homology for integration into 
                     B. amyloliquefaciens"
     CDS             complement(3263..3913)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetylase"
                     /label=cat
                     /note="chloramphenicol acetylase; gene=cat - 
                     note=chloramphenicol resistance - codon_start=1 - 
                     transl_table=11 - product=chloramphenicol acetylase - 
                     protein_id=AAB40300.1 - db_xref=GI:1185567 - 
                     translation=MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQ - 
                     EGYKFYPAFIFLVTRVINSNTAFRT"
                     /protein_id="APU90130.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(3263..3913)
                     /gene="cat"
                     /label=cat
     regulatory      4223..4364
                     /regulatory_class="promoter"
                     /note="PdfnA; promoter region of dfnA from B. 
                     amyloliquefaciens FZB42"
     5UTR           4372..4688
                     /note="dfnA leader region (truncated); truncated dfnA 
                     leader region missing intrinsic terminator sequence"
     regulatory      4691..4737
                     /regulatory_class="terminator"
                     /note="T7 terminator; transcription terminator for 
                     bacteriophage T7 RNA polymerase"
     terminator      4691..4737
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      4739..4784
                     /regulatory_class="terminator"
                     /note="lambda tR2 terminator; transcription terminator tR2 
                     from lambda phage"
     regulatory      4785..4830
                     /regulatory_class="terminator"
                     /note="rrnB T1 terminator; transcription terminator T1 from
                     the E. coli rrnB gene"
     terminator      4785..4830
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             4860..5576
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="YFP"
                     /protein_id="APU90131.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      5583..5616
                     /regulatory_class="terminator"
                     /note="YFP_terminator"
     CDS             complement(5690..6220)
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label=loaP
                     /note="LoaP; LoaP NusG-like antiterminator protein"
                     /protein_id="APU90132.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            complement(5690..6220)
                     /gene="loaP"
                     /label=loaP
     CDS             complement(6249..6308)
                     /codon_start=1
                     /gene="mini-xylA"
                     /product="XylA fragment"
                     /label=mini-xylA
                     /note="mini xylA ORF"
                     /protein_id="APU90133.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            complement(6249..6308)
                     /gene="mini-xylA"
                     /label=mini-xylA
                     /note="xylA"
     regulatory      complement(6378..6457)
                     /regulatory_class="promoter"
                     /note="PxylA; PxylA Xylose-Inducible Promoter"
     CDS             6669..7721
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label=xylR
                     /note="XylR"
                     /protein_id="APU90134.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            6669..7721
                     /gene="xylR"
                     /label=xylR
     misc_feature    7729..8628
                     /pseudo
                     /note="amyE; amyE front; amyE homology for integration into
                     B. amyloliquefaciens"
     regulatory      8848..8952
                     /regulatory_class="promoter"
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        8848..8952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8953..9813
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampR; gene=bla - note=ampicillin resistance - 
                     codon_start=1 - transl_table=11 - product=beta-lactamase - 
                     protein_id=AAB40303.1 - db_xref=GI:1185570 - 
                     translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY - 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAG"
                     /protein_id="APU90135.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            8953..9813
                     /gene="bla"
                     /label=bla
     rep_origin      9984..10572
                     /direction=RIGHT
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin 
                     of replication"